Key features and details
- Rabbit polyclonal to GABRB1 (phospho S434)
- Suitable for: ELISA, IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GABRB1 (phospho S434) antibody
See all GABRB1 primary antibodies
DescriptionRabbit polyclonal to GABRB1 (phospho S434)
Ab51123 detects endogenous levels of GABRB1 only when phosphorylated at serine 434.
Tested applicationsSuitable for: ELISA, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human GABRB1.
- Human breast carcinoma tissue or extracts from cos7 cells.
This product was previously labelled as GABA A Receptor beta 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab51123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).|
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- AW061132 antibody
- B230208N19Rik antibody
- GABA(A) receptor beta 1 antibody
All lanes : Anti-GABRB1 (phospho S434) antibody (ab51123)
Lane 1 : Extracts from cos7 cells with immunising peptide
Lane 2 : Extracts from cos7 cells with no peptide
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
This image shows paraffin-embedded human breast carcinoma tissue stained with ab51123 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
ICC/IF image of ab51123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51123 has been referenced in 1 publication.
- Kim JH et al. Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth. PLoS One 9:e102893 (2014). IHC-P ; Human . PubMed: 25047033