Key features and details
- Mouse monoclonal [N151/3] to GABRD
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Product nameAnti-GABRD antibody [N151/3]
See all GABRD primary antibodies
DescriptionMouse monoclonal [N151/3] to GABRD
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow
- Rat brain lysate
The clone number has been updated from S151-3 to N151/3, both clone numbers name the same antibody clone.
This product was previously labelled as GABA A Receptor delta
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Involvement in diseaseDefects in GABRD are the cause of susceptibility to generalized epilepsy with febrile seizures plus type 5 (GEFS+5) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
Defects in GABRD are the cause of susceptibility to idiopathic generalized epilepsy type 10 (IGE10) [MIM:613060]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Defects in GABRD are the cause of susceptibility to juvenile myoclonic epilepsy type 7 (EJM7) [MIM:613060]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRD sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- GABA(A) receptor subunit delta antibody
- Gabrd antibody
- Gamma aminobutyric acid GABA A receptor delta antibody
Anti-GABRD antibody [N151/3] (ab93619) at 1/1000 dilution + Rat cell line lysates at 15 µg
Sheep Anti-Mouse IgG HRP
Predicted band size: 51 kDa
Block: 15% BSA for 30 minutes at RT
Cold 100% methanol for 10 minutes at -20°C fixed human HaCaT cells labeling GABRD (green) using ab93619 at 1/10 dilution in ICC/IF.
FITC Goat Anti-Mouse was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. STaining in the diffuse cytoplasm and dull nuclei is observed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93619 has not yet been referenced specifically in any publications.