Overview

  • Product name

    Anti-GABRD antibody [N151/3]
    See all GABRD primary antibodies
  • Description

    Mouse monoclonal [N151/3] to GABRD
  • Host species

    Mouse
  • Specificity

    GABRD

  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Rat GABRD aa 15-34.
    Sequence:

    CTQPHHGARAMNDIGDYVGSN

  • Positive control

    • Rat brain lysate
  • General notes

    The clone number has been updated from S151-3 to N151/3, both clone numbers name the same antibody clone.

     

     This product was previously labelled as GABA A Receptor delta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab93619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notes
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 51 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
    • Involvement in disease

      Defects in GABRD are the cause of susceptibility to generalized epilepsy with febrile seizures plus type 5 (GEFS+5) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
      Defects in GABRD are the cause of susceptibility to idiopathic generalized epilepsy type 10 (IGE10) [MIM:613060]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
      Defects in GABRD are the cause of susceptibility to juvenile myoclonic epilepsy type 7 (EJM7) [MIM:613060]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
    • Sequence similarities

      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRD sub-subfamily.
    • Cellular localization

      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • GABA(A) receptor subunit delta antibody
      • Gabrd antibody
      • Gamma aminobutyric acid GABA A receptor delta antibody
      • Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody
      • Gamma-aminobutyric acid receptor subunit delta antibody
      • GBRD_HUMAN antibody
      • MGC45284 antibody
      see all

    Images

    • Anti-GABRD antibody [N151/3] (ab93619) at 1/1000 dilution + Rat cell line lysates at 15 µg

      Secondary
      Sheep Anti-Mouse IgG HRP

      Predicted band size: 51 kDa



      Block: 15% BSA for 30 minutes at RT

    • Cold 100% methanol for 10 minutes at -20°C fixed human HaCaT cells labeling GABRD (green) using ab93619 at 1/10 dilution in ICC/IF.

      FITC Goat Anti-Mouse was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. STaining in the diffuse cytoplasm and dull nuclei is observed.

    References

    ab93619 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain, cerebellar cortex)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton-X
    Specification
    brain, cerebellar cortex
    Blocking step
    (agent) for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde

    Prof. Marshall Devor

    Verified customer

    Submitted May 28 2019

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Mrs. Olga Cravetchi

    Verified customer

    Submitted Jul 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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