• Product name

  • Description

    Rabbit polyclonal to GABRP
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to GABRP aa 28-77 (N terminal).


    (Peptide available as ab206287)

  • Positive control

    • Jurkat whole cell lysate (ab7899) for Western blot, and Human intestine for Immunohistochemistry.
  • General notes

     This product was previously labelled as GABA A receptor pi




Our Abpromise guarantee covers the use of ab26055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with GABRP peptide (ab206287). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/312500.
ICC/IF Use at an assay dependent concentration. PubMed: 22700823


  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. In the uterus, the function of the receptor appears to be related to tissue contractility. The binding of this pI subunit with other GABA(A) receptor subunits alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone.
  • Tissue specificity

    Most abundant in the uterus, also expressed in lung, thymus and prostate.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GABA(A) receptor subunit pi antibody
    • GABRP antibody
    • Gamma aminobutyric acid (GABA) A receptor pi antibody
    • Gamma aminobutyric acid A receptor pi antibody
    • Gamma aminobutyric acid receptor pi subunit antibody
    • Gamma aminobutyric acid receptor subunit pi antibody
    • Gamma-aminobutyric acid receptor subunit pi antibody
    • GBRP_HUMAN antibody
    • MGC126386 antibody
    • MGC126387 antibody
    see all


  • Anti-GABRP antibody (ab26055) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 50 kDa
    Observed band size: >90,50 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 10%.
  • Ab26055 at 4-8 ug/ml staining GABRP in paraffin embedded human intestine by Immunohistochemistry. Arrows indicate the GABRP positive epithelial cells of intestinal villus. Magnification: x400.


This product has been referenced in:

  • Kaneko Y  et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016). WB ; Rat . Read more (PubMed: 27767042) »
  • Lu J  et al. GABA A receptor p subunit promotes apoptosis of HTR-8/SVneo trophoblastic cells: Implications in preeclampsia. Int J Mol Med 38:105-12 (2016). ICC, IHC ; Human . Read more (PubMed: 27221053) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (MDA-MB231 cell line)
Loading amount
20 µg
MDA-MB231 cell line
100µM GABA for 24hrs
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 13 2012


Thank you for your enquiry. Following communication with the source of this antiserum I have managed to find the protocol that was used to verify this antibody by IHC-P. I have uploaded it to our web site and it is available by clicking on the link on the antibody's datasheet or by clicking on the link below: https://www.abcam.com/index.html?pageconfig=protocols&pid=336&intAbID=26055&strTab=protocols&mode=prot I hope this information helps. Good luck!

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Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I have examined the EXPASY database for the expression of GABA A receptor pi and it appears that the protein is most abundant in the uterus and also expressed in lung, thymus and prostate. Therefore lung is a good choice of tissue for this antibody. However, to my knowledge this antibody has not been tested using rat cells. Therefore the cross reactivity that you are observing against the nuclei and absence of staining against the cell membrane may be a consequence of the absence of species reactivity. I would recommend using a positive control of human intestine if at all possible, performed in parallel with your rat lung. However, should this be possible I would advise a time course experiment of antigen retrieval so that you are presenting the antibody with the best opportunity to recognise its antigen. I am going to contact the source of this antibody to determine whether I can obtain more details regarding how the IHC was performed. I will be in touch with any information that transpires. I appreciate your patience in this matter.

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