The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:312500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 1 µg/ml.
GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the gaba/benzodiazepine receptors and opening an integral chloride channel. GABRR2 is a member of the rho subunit family of GABA receptors and could play a role in retinal neurotransmission.
ICC/IF image of ab83223 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83223, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Western blot - Anti-GABRR2 antibody (ab83223)
Anti-GABRR2 antibody (ab83223) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa Observed band size: 54 kDa
Bhandage AK et al. GABA Regulates Release of Inflammatory Cytokines From Peripheral Blood Mononuclear Cells and CD4+ T Cells and Is Immunosuppressive in Type 1 Diabetes. EBioMedicine30:283-294 (2018).
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Rosas-Arellano A et al. A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays. Histochem Cell Biol146:421-30 (2016).
Read more (PubMed: 27188756) »