Product nameAnti-GABRR2 antibody
See all GABRR2 primary antibodies
DescriptionRabbit polyclonal to GABRR2
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- WB: HeLa cell lysate ICC/IF: SKNSH cell line.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:312500.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use a concentration of 1 µg/ml.|
RelevanceGABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the gaba/benzodiazepine receptors and opening an integral chloride channel. GABRR2 is a member of the rho subunit family of GABA receptors and could play a role in retinal neurotransmission.
Cellular localizationCell junction > synapse > postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
- GABA receptor Rho 2 subunit antibody
- GABA(A) receptor antibody
- GABA(A) receptor subunit rho-2 antibody
ICC/IF image of ab83223 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83223, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-GABRR2 antibody (ab83223) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Gel Concentration: 12%
This product has been referenced in:
- Bhandage AK et al. GABA Regulates Release of Inflammatory Cytokines From Peripheral Blood Mononuclear Cells and CD4+ T Cells and Is Immunosuppressive in Type 1 Diabetes. EBioMedicine 30:283-294 (2018). WB . Read more (PubMed: 29627388) »
- Rosas-Arellano A et al. A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays. Histochem Cell Biol 146:421-30 (2016). Read more (PubMed: 27188756) »