Key features and details
- Mouse monoclonal [GAD1/2391] to GAD1
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-GAD1 antibody [GAD1/2391]
See all GAD1 primary antibodies
DescriptionMouse monoclonal [GAD1/2391] to GAD1
Tested applicationsSuitable for: Protein Array, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human GAD1 aa 72-135. The exact sequence is proprietary.
Database link: Q99259
- WB: Human brain lysate. IHC-P: Human brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab237998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.|
FunctionCatalyzes the production of GABA.
Tissue specificityIsoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Involvement in diseaseDefects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
Sequence similaritiesBelongs to the group II decarboxylase family.
- Information by UniProt
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1_HUMAN antibody
ab237998 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Anti-GAD1 antibody [GAD1/2391] (ab237998) at 1 µg/ml + Human brain lysate
Predicted band size: 67 kDa
Formalin-fixed, paraffin-embedded human brain tissue stained for GAD1 with ab237998 at 2 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237998 has not yet been referenced specifically in any publications.