Overview

  • Product name

    Anti-GAD1 antibody [GAD1/2563]
    See all GAD1 primary antibodies
  • Description

    Mouse monoclonal [GAD1/2563] to GAD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GAD1 aa 72-135. The exact sequence is proprietary.
    Database link: Q99259

  • Positive control

    • IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab237999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Protein Array Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the production of GABA.
  • Tissue specificity

    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in disease

    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similarities

    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1_HUMAN antibody
    • FLJ45882 antibody
    • GAD 1 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • GAD67 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041054 antibody
    • OTTHUMP00000041055 antibody
    • OTTHUMP00000228572 antibody
    • SCP antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human brain tissue stained for GAD1 using ab237999 at 2 µg/mL in immunohistochemical analysis.

  • ab237999 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

References

ab237999 has not yet been referenced specifically in any publications.

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