Anti-GAD65 + GAD67 antibody (ab11070)
Key features and details
- Rabbit polyclonal to GAD65 + GAD67
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-GAD65 + GAD67 antibody
See all GAD65 + GAD67 primary antibodies -
Description
Rabbit polyclonal to GAD65 + GAD67 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Pig
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Immunogen
Synthetic peptide:
DFLIEEIERLGQDL
, corresponding to C terminal amino acids 572-585 of Human GAD65+67. -
Positive control
- WB: Mouse brain tissue lysate.
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General notes
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. Store undiluted. The product can be stored for up to 12 months. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
An immunoglobulin-rich fraction was prepared from whole serum by ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65, 67 kDa (predicted molecular weight: 65 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 65, 67 kDa (predicted molecular weight: 65 kDa). |
Target
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Relevance
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
Cellular localization
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
Database links
- Entrez Gene: 14415 Mouse
- Entrez Gene: 14417 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 396929 Pig
- SwissProt: P48318 Mouse
- SwissProt: P48320 Mouse
- SwissProt: 48319 Pig
- SwissProt: P48321 Pig
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Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
Images
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Western blot - Anti-GAD65 + GAD67 antibody (ab11070)All lanes : Anti-GAD65 + GAD67 antibody (ab11070) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate (negative control)
Secondary
All lanes : Goat Anti-rabbit IgG (HRP)
Predicted band size: 65 kDa
Observed band size: 65,67 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (50)
ab11070 has been referenced in 50 publications.
- Chen M et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578
- Kasaragod VB et al. Pyridoxal kinase inhibition by artemisinins down-regulates inhibitory neurotransmission. Proc Natl Acad Sci U S A 117:33235-33245 (2020). PubMed: 33318193
- Ramaswamy M et al. Identification of GABAergic neurons innervating the zebrafish lateral habenula. Eur J Neurosci 52:3918-3928 (2020). PubMed: 32464693
- Galera-Monge T et al. Mitochondrial Dysfunction and Calcium Dysregulation in Leigh Syndrome Induced Pluripotent Stem Cell Derived Neurons. Int J Mol Sci 21:N/A (2020). PubMed: 32366037
- Kourgiantaki A et al. Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury. NPJ Regen Med 5:12 (2020). PubMed: 32566251