• Product name
    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD65 + GAD67
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human, Pig
  • Immunogen

    Synthetic peptide:


    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585).

  • Positive control
    • Rat brain extract. Rat pancreas tissue sections.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab49832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/1000.


  • Relevance
    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.
  • Cellular localization
    Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Database links
  • Alternative names
    • 65 kDa glutamic acid decarboxylase antibody
    • 67 kDa glutamic acid decarboxylase antibody
    • GAD 65 antibody
    • GAD 67 antibody
    • GAD1 antibody
    • GAD2 antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate decarboxylase 2 pancreatic islets and brain 65kDa antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • SCP antibody
    see all


  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.

  • ab49832 staining human pancreas tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C.  An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

    See Abreview


This product has been referenced in:
  • Tan W  et al. Neuronal loss in anterior cingulate cortex in spared nerve injury model of neuropathic pain. Neurochem Int 118:127-133 (2018). IHC-P . Read more (PubMed: 29894700) »
  • Kami K  et al. Improvements in impaired GABA and GAD65/67 production in the spinal dorsal horn contribute to exercise-induced hypoalgesia in a mouse model of neuropathic pain. Mol Pain 12:N/A (2016). Read more (PubMed: 27030712) »

See all 14 Publications for this product

Customer reviews and Q&As

Immunohistochemistry free floating
Rat Tissue sections (brain)

Arantxa Blasco

Verified customer

Submitted Apr 07 2018

IHC - Wholemount
Rat Tissue (Inhibitory Neurons)
Inhibitory Neurons

Abcam user community

Verified customer

Submitted Nov 18 2015

Thank you for contacting us. We recommend that you use this antibody for IHC-P at a dilution of 1/1000, incuabted overnight. You should first block the tissue with 10% normal serum plus 1% BSA for 1-2 hours to diminish background.

I hope thi...

Read More

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable....

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Thank you for contacting us.
I am sorry to hearthat you are encountering some problems with this antibody.

As discussed over the phone, I am sending the link to our antigen retrieval protocols:


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I am happy you received the antibody already, and hope it works as expected.
If not, please do let us know and we will be happy to assist you further.

Thank you for contacting us.

The anti-GAD65 + GAD67 antibody (ab49832) has to our knowledge not been used with free floating sections as yet and your customer would therefore be eligible for a testing discount. I have generated the code...

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As requested, I have issued a free of charge replacement with the order number XXXXXX from lot XXXXX of ab45429.

To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vi...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry free floating
Rat Tissue sections (Brain)

Abcam user community

Verified customer

Submitted Sep 22 2010

1-10 of 12 Abreviews or Q&A


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