Anti-GAD65 + GAD67 antibody (ab49832)
Rabbit polyclonal GAD65 + GAD67 antibody. Validated in WB, IHC and tested in Mouse, Rat, Cat, Human, Pig. Cited in 16 publication(s). Independently reviewed in 5 review(s).
- Datasheet
- References (19)
- Protocols
Overview
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Product name
Anti-GAD65 + GAD67 antibody
See all GAD65 + GAD67 primary antibodies -
Description
Rabbit polyclonal to GAD65 + GAD67 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cat, Human, Pig -
Immunogen
Synthetic peptide:
K-DIDFLIEEIERLGQDL
conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585). -
Positive control
- Rat brain extract. Rat pancreas tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab49832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Predicted molecular weight: 65 kDa. | |
| IHC-P | 1/1000 - 1/2000. |
Target
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Relevance
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
Cellular localization
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
Database links
- Entrez Gene: 2571 Human
- Entrez Gene: 2572 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 14417 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 396929 Pig
- Entrez Gene: 24379 Rat
- Entrez Gene: 24380 Rat
see all -
Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)This image is courtesy of an anonymous Abreviewab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
References
This product has been referenced in:
- Carballo-Carbajal I et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat Commun 10:973 (2019). Read more (PubMed: 30846695) »
- Tan W et al. Neuronal loss in anterior cingulate cortex in spared nerve injury model of neuropathic pain. Neurochem Int 118:127-133 (2018). IHC-P . Read more (PubMed: 29894700) »
