Anti-GAD65 + GAD67 antibody (ab49832)

Rabbit polyclonal GAD65 + GAD67 antibody. Validated in WB, IHC and tested in Mouse, Rat, Cat, Human, Pig. Cited in 16 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description

    Rabbit polyclonal to GAD65 + GAD67
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cat, Human, Pig
  • Immunogen

    Synthetic peptide:

    K-DIDFLIEEIERLGQDL

    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585).

  • Positive control

    • Rat brain extract. Rat pancreas tissue sections.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/1000.

Target

  • Relevance

    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.
  • Cellular localization

    Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Database links

  • Alternative names

    • 65 kDa glutamic acid decarboxylase antibody
    • 67 kDa glutamic acid decarboxylase antibody
    • GAD 65 antibody
    • GAD 67 antibody
    • GAD1 antibody
    • GAD2 antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate decarboxylase 2 pancreatic islets and brain 65kDa antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • SCP antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.

  • ab49832 staining human pancreas tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C.  An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Carballo-Carbajal I  et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat Commun 10:973 (2019). Read more (PubMed: 30846695) »
  • Tan W  et al. Neuronal loss in anterior cingulate cortex in spared nerve injury model of neuropathic pain. Neurochem Int 118:127-133 (2018). IHC-P . Read more (PubMed: 29894700) »
See all 19 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-5 of 5 Abreviews

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (brain)
Specification
brain

Arantxa Blasco

Verified customer

Submitted Apr 07 2018

Application
IHC - Wholemount
Sample
Rat Tissue (Inhibitory Neurons)
Specification
Inhibitory Neurons

Abcam user community

Verified customer

Submitted Nov 18 2015

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Sep 22 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO buffer
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 11 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No

Abcam user community

Verified customer

Submitted May 23 2008

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