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Can you inform me how I can do titration and dilution to reach optimal working dilution to make good contrast with no background and which diluent I use?name it please
Iam working IHC- P section of mouse pancreas using Anti-GAD65 + GAD67 antibody ab-49832.
Asked on Oct 18 2012
Thank you for contacting us. We recommend that you use this antibody for IHC-P at a dilution of 1/1000, incuabted overnight. You should first block the tissue with 10% normal serum plus 1% BSA for 1-2 hours to diminish background.
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Answered on Oct 18 2012