Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/200
Additional data
Additional Notes
Worked great with immunofluorescence on FFPE sections. Harder to optimize with HRP-DAB detection method. Too much background with biotinylated secondary-strepavidin method. Better with direct enzyme conjugation secondary antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 23 2008