Immunohistochemistry - Free Floating abreview for Anti-GAD65 + GAD67 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS containing 0.2% Triton-X

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
We used this antibody by itself without antigen retrieval using a standard IHC protocol on 10µm free-floating midbrain sections, which gave us unspecific staining, e.g. far more cells than expected were stained.
We repeated the protocol using an antigen retrieval protocol sodium citrate, this gave us no staining of cell bodies or dendrites at all.
Previously we used the ab108 and ab5992 using the same protocols with satisfactory results.
Abcam response
This customer received a testing discount to trial this antibody in IHC-FrFl. It may be that optimization is required, but Abcam's recommended free-floating protocol can be found here:

https://www.abcam.com/index.html?rid=189

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Verified customer

Submitted Sep 22 2010

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