Anti-GAD65 antibody (ab203063)

Rabbit polyclonal GAD65 antibody. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 2 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to GAD65
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GAD65 aa 541-567 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    GTTMVSYQPL GDKVNFFRMV ISNPAA


    Database link: Q05329

  • Positive control

    • Rat brain and testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab203063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Catalyzes the production of GABA.
  • Sequence similarities

    Belongs to the group II decarboxylase family.
  • Post-translational
    modifications

    Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.
  • Cellular localization

    Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt
  • Database links

  • Alternative names

    • 65 kDa glutamic acid decarboxylase antibody
    • DCE 2 antibody
    • DCE2 antibody
    • DCE2_HUMAN antibody
    • GAD 2 antibody
    • GAD 65 antibody
    • GAD-2 antibody
    • GAD-65 antibody
    • Gad2 antibody
    • Glutamate decarboxylase 2 (pancreas) antibody
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate Decarboxylase 65 antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamic Acid Decarboxylase 2 antibody
    • Glutamic Acid Decarboxylase 65 antibody
    • MGC161605 antibody
    • MGC161607 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat testis tissue labeling GAD65 with ab203063 at 1/200 dilution.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling GAD65 with ab203063 at 1/200 dilution.

References

This product has been referenced in:

  • Tan W  et al. Neuronal loss in anterior cingulate cortex in spared nerve injury model of neuropathic pain. Neurochem Int 118:127-133 (2018). WB . Read more (PubMed: 29894700) »
  • Gonzalez-Riano C  et al. Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus. Brain Struct Funct 222:2831-2853 (2017). Read more (PubMed: 28285370) »
See all 2 Publications for this product

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