Anti-GAD65 antibody [GAD-6] (ab26113)
Key features and details
- Mouse monoclonal [GAD-6] to GAD65
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-GAD65 antibody [GAD-6]
See all GAD65 primary antibodies -
Description
Mouse monoclonal [GAD-6] to GAD65 -
Host species
Mouse -
Specificity
The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Mammals -
Immunogen
Full length protein (Rat)
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Epitope
ab26113 recognizes an epitope near the C-terminus of GAD65 -
Positive control
- mouse brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
GAD-6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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Notes |
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WB
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
Target
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Function
Catalyzes the production of GABA. -
Sequence similarities
Belongs to the group II decarboxylase family. -
Post-translational
modificationsPhosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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Database links
- Entrez Gene: 14417 Mouse
- SwissProt: P48320 Mouse
- Unigene: 4784 Mouse
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Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab26113 has been referenced in 70 publications.
- Cheng YC et al. Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation. Stem Cell Rev Rep 18:839-852 (2022). PubMed: 35061207
- Zhu X et al. BRN2 as a key gene drives the early primate telencephalon development. Sci Adv 8:eabl7263 (2022). PubMed: 35245119
- Huang R et al. Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depression. J Physiol 600:2973-2999 (2022). PubMed: 35639046
- Koebele SV et al. Evaluating the Cognitive Impacts of Drospirenone, a Spironolactone-Derived Progestin, Independently and in Combination With Ethinyl Estradiol in Ovariectomized Adult Rats. Front Neurosci 16:885321 (2022). PubMed: 35692432
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866