Overview

  • Product name
    Anti-GAD65 antibody [GAD-6]
    See all GAD65 primary antibodies
  • Description
    Mouse monoclonal [GAD-6] to GAD65
  • Host species
    Mouse
  • Specificity
    The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammals
  • Immunogen

    Full length protein (Rat)

  • Epitope
    ab26113 recognizes an epitope near the C-terminus of GAD65
  • Positive control
    • mouse brain

Properties

Applications

Our Abpromise guarantee covers the use of ab26113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 18467596
WB 1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/100. PubMed: 24246263
ICC/IF Use at an assay dependent concentration. PubMed: 22745816

Target

  • Function
    Catalyzes the production of GABA.
  • Sequence similarities
    Belongs to the group II decarboxylase family.
  • Post-translational
    modifications
    Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.
  • Cellular localization
    Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 65 kDa glutamic acid decarboxylase antibody
    • DCE 2 antibody
    • DCE2 antibody
    • DCE2_HUMAN antibody
    • GAD 2 antibody
    • GAD 65 antibody
    • GAD-2 antibody
    • GAD-65 antibody
    • Gad2 antibody
    • Glutamate decarboxylase 2 (pancreas) antibody
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate Decarboxylase 65 antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamic Acid Decarboxylase 2 antibody
    • Glutamic Acid Decarboxylase 65 antibody
    • MGC161605 antibody
    • MGC161607 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [GAD-6] (ab26113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [GAD-6] (ab26113)
    Mouse monoclonal [GAD-6] to GAD65 staining GAD65-containing nerve terminals around cell bodies of Purkinje cells in the rat cerebellar cortex.
  • Western blot - Anti-GAD65 antibody [GAD-6] (ab26113)
    Western blot - Anti-GAD65 antibody [GAD-6] (ab26113)
    Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution + mouse cerebellum at 0.75 µg

    Secondary
    HRP horse anti-mouse IgG at 1/5000 dilution

    Predicted band size: 65 kDa

  • Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [GAD-6] (ab26113)
    Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [GAD-6] (ab26113)Winpenny et al, Neural Development, 6, 12, Fig. 4f.; doi: 10.1186/1749-8104-6-12

    Immunohistochemical analysis of frozen section from Mouse embryo, staining the glomerular layer of the brain. GAD65 is labelled with ab26113 (green) diluted 1:1000. Trb2 is labelled with ab23345 (red).

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GAD65 antibody [GAD-6] (ab26113)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GAD65 antibody [GAD-6] (ab26113)This image is courtesy of an Abreview submitted by Patrick Ye.

    Immunohistochemical analysis of Mouse brain cortex sections, labelling GAD65 with ab26113, diluted 1/100.

    See Abreview

References

This product has been referenced in:
  • Woodhouse A  et al. Repeat propofol anesthesia does not exacerbate plaque deposition or synapse loss in APP/PS1 Alzheimer's disease mice. BMC Anesthesiol 18:47 (2018). Read more (PubMed: 29699479) »
  • Hwang S  et al. Hypoxia regulates the level of glutamic acid decarboxylase enzymes and interrupts inhibitory synapse stability in primary cultured neurons. Neurotoxicology 65:221-230 (2018). WB . Read more (PubMed: 29107683) »

See all 32 Publications for this product

Publishing research using ab26113? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

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Western blot abreview for Anti-GAD65 antibody [GAD-6]

 Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (human hippocampus)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Treatment
used protease inhibitors
Specification
human hippocampus
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
Username

Abcam user community

Verified customer

Submitted Jun 25 2015



Inquiry: Hello, Do you have an antigen for pre-adsorption controls for teh following product? https://www.abcam.com/GAD65-antibody-GAD-6-Neuronal-Marker-ab26113.html

Thank you for the clarification! Unfortunately the blocking peptide is not available for this particular antibody. Please let me know if I can be of further assistance.


Thank you for replacement GAD65 ab 112497. I try it on my western blot. The ab
lind up to all my mark. and no band was found on my positive control(Rat brain
tissue). The reason I select 112497, because I think this is polycolonal ab and...

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Thank you very much for your reply.

I am sorry to hear that the replacement ab112497 did not work in Western blot. Just to confirm, there were multiple bands in your neuron lysate, and no bands with the rab brain lysate? Were you using the sa...

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Thank you for your information.
I didn't try it in other Rat tissues, I may try it and let you know the results.
I also couldn't find any related papers.
I would like to try polycolone Ab.
Best Regards!

Thank you very much for your reply.

I'm sending a free of charge vial of ab112497 on the order **** so that you can compare the results to those with ab26113. This antibody is out of stock but we expect it to arrive around September 27 (next ...

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Band around 65 kDa with rat brain tissue lysate, 40 kDa in neural cell lysate. Is this 40 kDa band specific. 1:1000 dilution. Normal culture conditions.

Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

I actually could not find a reference showing analysis of endogneous GAD65 in culture neurons by Western blot. So, I'm still not quite sure...

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Hello,



thanks a lot for your answer.

I would have another question though: as the GAD65 antibody is confined to nerve terminals, is it (in general) possible to stain for GAD65 cell bodies? Are there antibodies that are...

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Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. W...

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Lot number: Gr56581-1
Inquiry: Hello, in our lab we want to stain for GAD65 neuronal cell bodies in mouse spinal cord (see protocol attached). As described in the literature and our protocol, we leave out any Triton steps for this. The staining wo...

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Thank you for your enquiry regarding ab26113.

It has been reported that GAD65 is transported to nerve terminals and this antibody (ab26113) should stain them as “puncta” around the cell body of their target neurons.

On t...

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DESCRIPTION OF THE PROBLEM No signal SAMPLE Neurodifferentiated human embryonic stem cells PRIMARY ANTIBODY Abcam (ab26113), Mouse monoclonal to GAD65, in Knouckout Serum replacement @ 1:500, incubate for 1 hour and wash 3 times with 100 uL PBS ++ DETE...

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Thank you for contacting Abcam.

Based on the protocol the customer sent, there are a few suggestions that may help resolve the issue:

1 - Increase the concentration of primary antibody used to 1/200.

2 - Increase the incub...

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