Anti-GAD65 antibody [GAD-6] (ab26113)
Key features and details
- Mouse monoclonal [GAD-6] to GAD65
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-GAD65 antibody [GAD-6]
See all GAD65 primary antibodies -
Description
Mouse monoclonal [GAD-6] to GAD65 -
Host species
Mouse -
Specificity
The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991). -
Tested Applications & Species
Application Species ICC Mouse -
Immunogen
Full length protein (Rat)
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Epitope
ab26113 recognizes an epitope near the C-terminus of GAD65 -
Positive control
- mouse brain
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
GAD-6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Mouse
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All applications |
Mammals
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Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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Notes |
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WB
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
Target
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Function
Catalyzes the production of GABA. -
Sequence similarities
Belongs to the group II decarboxylase family. -
Post-translational
modificationsPhosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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Database links
- Entrez Gene: 14417 Mouse
- SwissProt: P48320 Mouse
- Unigene: 4784 Mouse
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Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
Images
Protocols
References (44)
ab26113 has been referenced in 44 publications.
- Shah M et al. Recurrent glucose deprivation leads to the preferential use of lactate by neurons in the ventromedial hypothalamus. Am J Physiol Endocrinol Metab 316:E948-E955 (2019). PubMed: 30888861
- Qin J et al. Early Forebrain Neurons and Scaffold Fibers in Human Embryos. Cereb Cortex N/A:N/A (2019). PubMed: 31298263
- Ge MM et al. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain. Eur J Pharmacol 858:172475 (2019). PubMed: 31228456
- Adaikkan C et al. Gamma Entrainment Binds Higher-Order Brain Regions and Offers Neuroprotection. Neuron 102:929-943.e8 (2019). PubMed: 31076275
- Zhu C et al. The Chinese Medicine Wu-Tou Decoction Relieves Neuropathic Pain by Inhibiting Hippocampal Microglia Activation. Sci Rep 8:12292 (2018). PubMed: 30115941