Question (63346) | Anti-GAD65 antibody [GAD-6] (ab26113)

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Question


Thank you for replacement GAD65 ab 112497. I try it on my western blot. The ab
lind up to all my mark. and no band was found on my positive control(Rat brain
tissue). The reason I select 112497, because I think this is polycolonal ab and
may give mpre chance for my cells.
If you can send me another GAD 65 ab 75750, I can try!
Thanks!

Answer

Thank you very much for your reply.

I am sorry to hear that the replacement ab112497 did not work in Western blot. Just to confirm, there were multiple bands in your neuron lysate, and no bands with the rab brain lysate? Were you using the same protocol as before?

I'm sending a replacement vial of ab75750 on the order ***, which we expect to arrive around Thursday of next week. Please keep me updated about the results with this antibody as well. If you have any questions or need anything else, please let me know and I'll be happy to help.

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