Western blot abreview for Anti-GAD65 antibody [GAD-6]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (human hippocampus)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Treatment
used protease inhibitors
Specification
human hippocampus
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Tris buffered saline, 3% BSA+0.1% Tween 20
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: horse anti-mouse
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL2
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 0 kDa
Positive control
recombinant purified GAD65

Additional data

Additional Notes
excellent results on human hippocampi western blot

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Submitted Jun 25 2015

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