Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GAD65 antibody [GAD-6]

Average
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain - cortex)
Antigen retrieval step
None
Permeabilization
Yes - 0.5% Triton-X in 10% goat serum
Specification
brain - cortex
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% goat serum with 0.5% Triton-X
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor anti-mouse 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
The staining using this antibody for thick sections can be difficult to interpret because the staining pattern is diffuse.

Patrick Ye

Verified customer

Submitted Dec 12 2016

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