Overview

  • Product name
    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD65 + GAD67
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cat, Human, Zebrafish, Macaque monkey
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:

    DFLIEEIERLGQDL

    , corresponding to C terminal amino acids 572-585 of Human GAD65+67.

  • Positive control
    • Recombinant Human GAD67 protein (ab114255) can be used as a positive control in WB. Brain.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    An immunoglobulin-rich fraction was prepared from whole serum by ammonium sulphate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20650017
IHC-Fr 1/200 - 1/5000.
WB 1/1000 - 1/10000. Predicted molecular weight: 65 , 67 kDa.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    Images

    • ab11070 staining GABA transporter 2 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab11070 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. GAD65/67 expression can be observed in amacrine cells (in green).

      See Abreview

    References

    This product has been referenced in:
    • Fukumura S  et al. Intravenous infusion of mesenchymal stem cells reduces epileptogenesis in a rat model of status epilepticus. Epilepsy Res 141:56-63 (2018). IHC . Read more (PubMed: 29475054) »
    • Kemp KC  et al. Aberrant cerebellar Purkinje cell function repaired in vivo by fusion with infiltrating bone marrow-derived cells. Acta Neuropathol 135:907-921 (2018). IHC ; Mouse . Read more (PubMed: 29541917) »
    See all 29 Publications for this product

    Customer reviews and Q&As

    1-10 of 12 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS Triton 3x5mn RT
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Fixative
    Paraformaldehyde

    Yacine Tensaouti

    Verified customer

    Submitted Aug 02 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Amacrine cells)
    Specification
    Retina, Amacrine cells
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde

    Dr. Ryan Macdonald

    Verified customer

    Submitted Sep 03 2013

    Answer

    Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

    All three of ourAnti-GAD65 + GAD67 antibodies are guaranteed to work in mouse tissues. Ab49832 and Ab11070 are both tested and guaranteed in IHC. I would be very happy to help you to find the best secondary antibody for use with either of these products. In order to determine this could you please let me know the detection system you plan to use?

    I am sorry that I could not be more helpful regarding the best marker for your needs, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

    Read More

    Answer

    Gracias por contactarnos de nuevo.

    Siento mucho los problemas ocasionados por estos productos que no han funcionado como se esperaba.

    Para poder dar el soporte oportuno de los mismos y entender por qué han dado ese resultado, te rogaría que por favor me ampliaras la siguiente información:

    - ¿Cuál es el numero de pedido de los anticuerpos?

    - ¿Cuáles son los lotes que funcionaron en el pasado de ab59791 y ab49917?

    - ¿En qué condiciones se ha llevado a cabo el lisado de tejidos (buffer, inhibidores, agente reductor, etc...)?

    - ¿Se ha usado un control positivo en paralelo con las muestras?

    - ¿Se ha comprobado el buen estado del secundario utilizado, así como del sustrato DAB? Es decir, ¿se han usado con otros sistemas obteniendo un resultado positivo?

    - Recomendamos llevar a cabo una incubación de anticuerpo de 12-16 horas para asegurar que el anticuerpo alcanza el epítopo.

    Por otro lado, quisiera asegurar al cliente que los anticuerpos no han podido afectarse por el hecho de llegarles a 8C. El envío del producto en los bloques azules de hielo es solo una medida de precaución, y ésta no es la primera vez que un cliente nos reporta la llegada de los productos a temperatura ambiente, sin que posteriormente haya sufrido algún problema con el mismo.

    En nuestros laboratorios hemos hecho estudios de estabilidad de los anticuerpos y proteínas con la temperatura, demostrando que son estables hasta a 45C durante una semana sin que pueda impactar en su actividad.

    El lunes estaré fuera de la oficina y no podre contestar los correos, pero a partir del martes día 2 podre segur adelante con esta reclamación. Espero tu respuesta entonces.

    Que pases un buen día.

    Read More

    Answer

    Thank you for your reply. I hope the increased antibody will help to label the cell bodies. If you are able to get specific signal, then you may be able to reduce the background by shortening your antigen retrieval or increasing the blocking time. If not, please let me know your original order or PO number and I will be happy to help you further.

    Read More

    Answer

    Thank you for contacting us. I am sorry that this antibody has been giving no signal in your IHC. Could you please clarify a few points of your protocol?

    1) What type of samples are you working with? What species and tissue type? How long were your samples fixed for?

    2) Did you observe any background signal or were the sections completely blank? Have you used your secondary antibody recently with other primary antibodies? What secondary antibody were you using?

    3)In some cases, long wash steps can reduce the antibody binding. I would recommend trying 3 x 5 minute washes after the primary and secondary antibody incubations.

    4)Depending on the expression of GAD65 + GAD 67 in your samples, you may need to use up to a 1:200 dilution for this primary antibody. Using more antibody may help to increase your signal.

    I hope this helps, if not, please let me know your original order or PO number and I will be happy to assist you further.

    Read More

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. I would like to confirm that you are eligible for our testing discount program if the antibody has not yet been purchased. If you are interested I will issue an 100% discount code for you. You would have to buy ab11070 and then test ab11070 with the mentioned recombinant proteins in WB. After submitting an Abreview the discount could would become active and you would be able to order your free antibody as an reward for testing this for future users and us at Abcam. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions or if your are interested in this offer.  

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Quail Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Ms. Charlotte Cornil

    Verified customer

    Submitted Sep 30 2011

    Answer

    Thank you for participating in our testing discount program. When you submit your abreivew, please be sure to note the testing discount code in the "additional notes" section. The website does have specific questions for you to answer as well as the notes section for any additional information you may have. Once you have submitted the review you will be able to use the code for an additional antibody. You will need to specify the code to the customer service agent when ordering so that you receive the antibody free. The testing discount program only provides a free antibody per each purchased antibody, so you would not be able to receive another code for this free antibody. Please let me know if you have any further questions.

    Read More

    1-10 of 12 Abreviews or Q&A

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