Overview

  • Product name

    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description

    Rabbit polyclonal to GAD65 + GAD67
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
    Unsuitable for: IHC-FoFr
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Cat, Human, Zebrafish, Macaque monkey
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:

    DFLIEEIERLGQDL

    , corresponding to C terminal amino acids 572-585 of Human GAD65+67.

  • Positive control

    • Recombinant Human GAD67 protein (ab114255) can be used as a positive control in WB. Brain.
  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    An immunoglobulin-rich fraction was prepared from whole serum by ammonium sulphate precipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20650017
IHC-Fr 1/200 - 1/5000.
WB 1/1000 - 1/10000. Predicted molecular weight: 65 , 67 kDa.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    Images

    • ab11070 staining GABA transporter 2 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab11070 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. GAD65/67 expression can be observed in amacrine cells (in green).

      See Abreview

    References

    This product has been referenced in:

    • Stevenson SA  et al. Intervention changes acoustic peak frequency and mesolimbic neurochemistry in the Pink1-/- rat model of Parkinson disease. PLoS One 14:e0220734 (2019). Read more (PubMed: 31374106) »
    • Samarut É  et al. ?-Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment. Epilepsia 59:2061-2074 (2018). Read more (PubMed: 30324621) »
    See all 35 Publications for this product

    Customer reviews and Q&As

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    1-5 of 5 Abreviews

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Monkey Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 0.2%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Mar 07 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS Triton 3x5mn RT
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 02 2017

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Amacrine cells)
    Specification
    Retina, Amacrine cells
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde

    Dr. Ryan Macdonald

    Verified customer

    Submitted Sep 03 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Quail Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Ms. Charlotte Cornil

    Verified customer

    Submitted Sep 30 2011

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (P5 neocortex (brain))
    Loading amount
    10 µg
    Specification
    P5 neocortex (brain)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

    Mr. Tim Sargeant

    Verified customer

    Submitted May 02 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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