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Thanks for your message.
I am working with mouse brains. So I perfused the animal with PBS followed by PFA, and then post fixed the brain o/n at 4C with 4% PFA.
I did observe some background, but there were no cell bodies labeled at all. So the sections were not blank.
I will take your suggestion about the antibody dilution and the wash steps and let you know how it goes.
Asked on Apr 30 2012
Thank you for your reply. I hope the increased antibody will help to label the cell bodies. If you are able to get specific signal, then you may be able to reduce the background by shortening your antigen retrieval or increasing the blocking time. If not, please let me know your original order or PO number and I will be happy to help you further.
Answered on Apr 30 2012