Immunohistochemistry (Frozen sections) abreview for Anti-GAD65 + GAD67 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Quail Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500

Additional data

Additional Notes
We also tested:
- dilution curve: 1/250, 1/500, 1/1000, 1/2000 and 1/5000
- fixation: PAF3% + Picric acid 15% (perfusion) PAF 4% + Picric Acid 15% + Glutaraldehyde 0.1% (perfusion) PAF 4% + Glutaraldehyde 0.1% (perfusion) acrolein 5% (immersion unmasking was performed with NaBH4 for 15 min as a first step of the protocol)
- incubation time from 16 to 96 hours

These different conditions did not yield any specific staining. Non specififc staining was minimal such that this conclusion is extremely obvious. The total absence of staining renders the presentation of a picture completely useless.
Abcam response
Thank you for your valuable feedback. We do not test this antibody in quail tissue, and the antibody may not be suitable for use with this species. The reviewer received a testing discount code to try this antibody in quail.

Ms. Charlotte Cornil

Verified customer

Submitted Sep 30 2011

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