We also tested: - dilution curve: 1/250, 1/500, 1/1000, 1/2000 and 1/5000 - fixation: PAF3% + Picric acid 15% (perfusion) PAF 4% + Picric Acid 15% + Glutaraldehyde 0.1% (perfusion) PAF 4% + Glutaraldehyde 0.1% (perfusion) acrolein 5% (immersion unmasking was performed with NaBH4 for 15 min as a first step of the protocol) - incubation time from 16 to 96 hours
These different conditions did not yield any specific staining. Non specififc staining was minimal such that this conclusion is extremely obvious. The total absence of staining renders the presentation of a picture completely useless.
Thank you for your valuable feedback. We do not test this antibody in quail tissue, and the antibody may not be suitable for use with this species. The reviewer received a testing discount code to try this antibody in quail.
Ms. Charlotte Cornil
Submitted Sep 30 2011
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