Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAD65 + GAD67 antibody

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS Triton 3x5mn RT
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s)
Dilution
1/500

Secondary antibody

Dilution
1/200

Additional data

Abcam response
This product is tested in Immunohistochemistry with paraffin embedded sections - for good results we can recommend trying antigen retrieval with buffers like EDTA or Citrate pH 6.0.

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Verified customer

Submitted Aug 02 2017

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