Key features and details
- Rabbit polyclonal to GAD67
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GAD67 antibody
See all GAD67 primary antibodies
DescriptionRabbit polyclonal to GAD67
Specificityab139393 detects endogenous levels of total GAD1 protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide from C terminal amino acids of Human GAD1 (NP_000808.2).
- Human small intestine and Human brain cortex tissues; extracts from LOVO cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 67 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the production of GABA.
Tissue specificityIsoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Involvement in diseaseDefects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
Sequence similaritiesBelongs to the group II decarboxylase family.
- Information by UniProt
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
All lanes : Anti-GAD67 antibody (ab139393) at 1/500 dilution
Lane 1 : extracts from LOVO cell
Lane 2 : extracts from LOVO cell treated with the synthesized peptide
Predicted band size: 67 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain, cortex tissue labeling GAD1 with ab139393 at 10 µg/ml.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human small intestine tissue labeling GAD1 with ab139393 at 10 µg/ml.
ab139393 has not yet been referenced specifically in any publications.