Overview

  • Product name

  • Description

    Rabbit polyclonal to GAD67
  • Host species

    Rabbit
  • Specificity

    ab139393 detects endogenous levels of total GAD1 protein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from C terminal amino acids of Human GAD1 (NP_000808.2).

  • Positive control

    • Human small intestine and Human brain cortex tissues; extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab139393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the production of GABA.
  • Tissue specificity

    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in disease

    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similarities

    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 1 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041054 antibody
    • OTTHUMP00000041055 antibody
    • OTTHUMP00000228572 antibody
    • SCP antibody
    see all

Images

  • All lanes : Anti-GAD67 antibody (ab139393) at 1/500 dilution

    Lane 1 : extracts from LOVO cell
    Lane 2 : extracts from LOVO cell treated with the synthesized peptide

    Predicted band size: 67 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain, cortex tissue labeling GAD1 with ab139393 at 10 µg/ml.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human small intestine tissue labeling GAD1 with ab139393 at 10 µg/ml.

References

ab139393 has not yet been referenced specifically in any publications.

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