Anti-GAD67 antibody (ab80589)

Goat polyclonal GAD67 antibody. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 1 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Goat polyclonal to GAD67
  • Host species

    Goat
  • Specificity

    ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cat, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-PDSPQRREKLHK

    , corresponding to internal sequence amino acids 526 - 537 of Human GAD67 (NP_000808.2)

  • Positive control

    • Recombinant Human GAD67 protein (ab114255) can be used as a positive control in WB. Mouse brain lysate, Rat brain lysate and Human brain (cerebellum) lysate. IHC-P: Human hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab80589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of GAD67 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80589, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-GAD67 antibody (ab80589) at 1 µg/ml + Mouse Brain lysate (in RIPA buffer) at 35 µg

    Predicted band size: 67 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Anti-GAD67 antibody (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

References

This product has been referenced in:

  • Ellis JK  et al. Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams. J Comp Neurol N/A:N/A (2019). Read more (PubMed: 31050805) »
  • Corbridge SM  et al. JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy. J Neurovirol N/A:N/A (2019). Read more (PubMed: 31025264) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS + 0.2% Triton X-100 + 4% BSA
Specification
Brain
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde

Dr. Alexandre Magno

Verified customer

Submitted Aug 02 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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