Recombinant Rabbit monoclonal [EPR20578] to GAD67/GAD1 (ab213508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20578] to GAD67/GAD1
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Rabbit monoclonal [EPR20578] to GAD67/GAD1
See all GAD67/GAD1 primary antibodies -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Common marmoset -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human cerebellum and fetal brain lysates; Mouse and rat cerebellum and brain lysates; IHC-P: Mouse and rat cerebral cortex, cerebellar cortex and pancreas tissues; IP: Human brain lysate; ICC/IF: Mouse primary neuron and rat midbrain neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20578 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50.
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WB |
1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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IHC-P | (3) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We don’t recommend this antibody for human in IHC. In our hands human tissues showed weak and non-specific staining. |
IP |
1/30.
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Notes |
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ICC/IF
1/50. |
WB
1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We don’t recommend this antibody for human in IHC. In our hands human tissues showed weak and non-specific staining. |
IP
1/30. |
Target
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Function
Catalyzes the production of GABA. -
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 24379 Rat
- Omim: 605363 Human
- SwissProt: Q99259 Human
- SwissProt: P48318 Mouse
- SwissProt: P18088 Rat
- Unigene: 420036 Human
see all -
Alternative names
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GAD1/GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GAD1/GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
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All lanes : Rabbit monoclonal [EPR20578] to GAD67/GAD1 (ab213508) at 1/1000 dilution
Lane 1 : Mouse cerebellum lysate
Lane 2 : Mouse lung lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat cerebellum lysate
Lane 5 : Rat lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Mouse lung, rat lung (PMID: 10671565).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling GAD1/GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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Immunohistochemical analysis of paraffin-embedded mouse cerebellar cortex tissue labeling GAD1/GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of mouse pancreas (PMID: 8243826). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of rat pancreas (PMID: 8243826). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Rabbit monoclonal [EPR20578] to GAD67/GAD1 (ab213508) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Human lung (PMID: 10671565).
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Rabbit monoclonal [EPR20578] to GAD67/GAD1 (ab213508) at 1/1000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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GAD67 was immunoprecipitated from 0.35 mg of human brain lysate with ab213508 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213508 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Human brain lysate 10 µg (Input).
Lane 2: ab213508 IP in human brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213508 in human brain lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab213508 has been referenced in 2 publications.
- Battaglia CR et al. Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons. Cell Death Dis 11:1004 (2020). PubMed: 33230105
- Li Y et al. Persistent Toxoplasma Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia. Infect Immun 87:N/A (2019). PubMed: 31182619