Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116)
Key features and details
- Mouse monoclonal [K-87] to GAD67/GAD1
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Mouse monoclonal [K-87] to GAD67/GAD1
See all GAD67/GAD1 primary antibodies -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human GAD1/GAD67 aa 50-150.
Database link: Q99259-1 -
Positive control
- Recombinant Human GAD67 protein (ab114255) can be used as a positive control in WB. In WB, this antibody gave a positive signal in Mouse, Rat, and Human brain tissue lysates. GAD67 has been thought to be primarily located in the nerve cell body, but using this new K-87 mAb, GAD67 can now also be detected in dendrites and axons.
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General notes
ab26116 mouse monoclonal [K-87] to GAD1/GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991). -
Clonality
Monoclonal -
Clone number
K-87 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
(methanol fixed cells) |
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IHC-P | (11) |
1/1000 - 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998).
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WB | (6) |
1/5000 - 1/100000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. (methanol fixed cells) |
IHC-P
1/1000 - 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998). |
WB
1/5000 - 1/100000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Catalyzes the production of GABA. -
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 24379 Rat
- Omim: 605363 Human
- SwissProt: Q99259 Human
- SwissProt: P48318 Mouse
- SwissProt: P18088 Rat
- Unigene: 420036 Human
see all -
Alternative names
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
Images
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ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.
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Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) at 1/2000 dilution + mouse cerebellum at 0.75 µg
Secondary
HRP horse anti-mouse IgG at 1/5000 dilution
Predicted band size: 67 kDa -
Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) at 1/100000 dilution + Human brain
Secondary
Rabbit anti-mouse antibody at 1/100000 dilution
Predicted band size: 67 kDa -
Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.
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ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (90)
ab26116 has been referenced in 90 publications.
- Forsthofer M & Straka H Homeostatic plasticity of eye movement performance in Xenopus tadpoles following prolonged visual image motion stimulation. J Neurol 270:57-70 (2023). PubMed: 35947153
- Ren D et al. Anterior Cingulate Cortex Mediates Hyperalgesia and Anxiety Induced by Chronic Pancreatitis in Rats. Neurosci Bull 38:342-358 (2022). PubMed: 34907496
- Zhou H et al. Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice. Mol Brain 15:53 (2022). PubMed: 35701839
- Ton HT et al. Early Thalamic Injury After Resuscitation From Severe Asphyxial Cardiac Arrest in Developing Rats. Front Cell Dev Biol 9:737319 (2021). PubMed: 34950655
- Humburg BA et al. Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice. Addict Biol 26:e13005 (2021). PubMed: 33538103