Western blot abreview for Anti-GADD34 antibody

Below Average
Abreviews
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver, muscle, mammary)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver, muscle, mammary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/20000
Name

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 73 kDa Non-specific: 30, 150 kDa

Additional data

Abcam response
We would like to thank this researcher for submitting their review. Upon investigation of this negative Abreview, we followed up with the customer with protocol advise to improve the western blot. GADD34 expression may be low in muscle and liver sample types.

Julie Kim

Verified customer

Submitted May 05 2017

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