Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab175355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/1000. Predicted molecular weight: 73 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionRecruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
Sequence similaritiesBelongs to the PPP1R15 family.
modificationsPhosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- Apoptosis associated protein antibody
- GADD 34 antibody
- Growth arrest and DNA damage inducible 34 antibody
Lane 2 : Anti-GADD34 antibody (ab175355) at 1/250 dilution
Lanes 3 & 5 : Anti-GADD34 antibody (ab175355) at 1/500 dilution
Lane 4 : Anti-GADD34 antibody (ab175355) at 1/1000 dilution
Lanes 2-4 : HeLa cell lysate
Lane 5 : HeLa cell lysate with neutralizing peptide
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP secondary antibody
Developed using the ECL technique.
Predicted band size: 73 kDa
Immunofluorescent analysis of formalin-fixed HeLa cells labeling GADD34 with ab175355 at 1/50 and incubated with DyLight 488 Goat anti-rabbit IgG secondary antibody. F-actin (red) was stained with DyLight 650 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
ab175355 has not yet been referenced specifically in any publications.