• Product name
  • Description
    Rabbit polyclonal to GADD45B
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 81-130 (FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW) of Human GADD45B (NP_056490).Peptide available as ab108448.

  • Positive control
    • HepG2 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung. IF/ICC: PANC-1 cell line



Our Abpromise guarantee covers the use of ab105060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 18 kDa.Can be blocked with GADD45B peptide (ab108448). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.


  • Function
    Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
  • Sequence similarities
    Belongs to the GADD45 family.
  • Information by UniProt
  • Database links
  • Form
    It localizes to the nucleus.
  • Alternative names
    • GA45B_HUMAN antibody
    • GADD45 Beta antibody
    • Gadd45b antibody
    • GADD45BETA antibody
    • Growth arrest and DNA damage inducible 45 beta antibody
    • Growth arrest and DNA damage inducible beta antibody
    • Growth arrest and DNA damage-inducible gene GADD45, beta antibody
    • Growth arrest and DNA damage-inducible protein GADD45 beta antibody
    • Growth arrest and DNA-damage-inducible protein GADD45 beta antibody
    • MY118 antibody
    • MYD118 antibody
    • MYD118, mouse, homolog of antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD118 antibody
    • Negative growth regulatory protein MyD118 antibody
    • Negative growth-regulatory protein MyD118 antibody
    see all


  • Anti-GADD45B antibody (ab105060) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size: 18 kDa

    Gel concentration: 12%
  • IHC image of GADD45B staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105060, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab105060 stained PANC-1 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105060, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • GADD45B was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to GADD45B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab105060.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 17kDa; non specific bands - 34 and 76kDa: We are unsure as to the identity of this extra band; GADD45B


This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.
Your credit note ID is XXXXX.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Thank you for your email.
In the original email Cell lytic M buffer was mentioned and in this RIPA buffer; could you confirm the buffer used?
Please also check the quality of blocking buffer and other chemicals?
I am sorry we do not have any further suggestions for protocol improvement. The antibodies should have worked. I am happy to refund your money you have paid for original antibody.
I hope this information is nevertheless helpful. Please do not hesitate to contact me with any further inquiries.

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