Anti-GAK antibody (ab186120)
Key features and details
- Rabbit polyclonal to GAK
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GAK antibody
See all GAK primary antibodies -
Description
Rabbit polyclonal to GAK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cat, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide within Human GAK aa 650-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14976 -
Positive control
- WB: GaMG, HeLa, HEK293T, Jurkat, RKO, CT26, CH27, and TCMK-1 Whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 143 kDa. |
Target
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Function
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. -
Tissue specificity
Ubiquitous. Highest in testis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 C2 tensin-type domain.
Contains 1 J domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus > trans-Golgi network. Cell junction > focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions. - Information by UniProt
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Database links
- Entrez Gene: 101094018 Cat
- Entrez Gene: 461048 Chimpanzee
- Entrez Gene: 100766883 Chinese hamster
- Entrez Gene: 479133 Dog
- Entrez Gene: 101129136 Gorilla
- Entrez Gene: 100051853 Horse
- Entrez Gene: 2580 Human
- Entrez Gene: 231580 Mouse
see all -
Alternative names
- Auxilin 2 antibody
- Cyclin G associated kinase antibody
- Cyclin-G-associated kinase antibody
see all
Images
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All lanes : Anti-GAK antibody (ab186120) at 1/1000 dilution
Lane 1 : GaMG Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : HEK293T Whole cell lysate
Lane 4 : Jurkat Whole cell lysate
Lane 5 : RKO Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 143 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-GAK antibody (ab186120) at 1/1000 dilution
Lane 1 : CT26 Whole cell lysate
Lane 2 : CH27 Whole cell lysate
Lane 3 : TCMK-1 Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 143 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186120 has been referenced in 1 publication.
- Karim M et al. Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for antiviral strategies. Antiviral Res 204:105367 (2022). PubMed: 35738348