Key features and details
- Rabbit polyclonal to GAK
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GAK antibody
See all GAK primary antibodies
DescriptionRabbit polyclonal to GAK
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cat, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human GAK aa 675-725. The exact sequence is proprietary.
Database link: 014976
- HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 143 kDa.|
FunctionAssociates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Tissue specificityUbiquitous. Highest in testis.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 C2 tensin-type domain.
Contains 1 J domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus > trans-Golgi network. Cell junction > focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.
- Information by UniProt
- Auxilin 2 antibody
- Cyclin G associated kinase antibody
- Cyclin-G-associated kinase antibody
All lanes : Anti-GAK antibody (ab186120) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 3 minutes
ab186120 has not yet been referenced specifically in any publications.