Product nameAnti-GAK antibody
See all GAK primary antibodies
DescriptionRabbit polyclonal to GAK
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cat, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human GAK aa 675-725. The exact sequence is proprietary.
Database link: 014976
- HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 143 kDa.|
FunctionAssociates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Tissue specificityUbiquitous. Highest in testis.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 C2 tensin-type domain.
Contains 1 J domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus > trans-Golgi network. Cell junction > focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.
- Information by UniProt
- Auxilin 2 antibody
- Cyclin G associated kinase antibody
- Cyclin-G-associated kinase antibody
All lanes : Anti-GAK antibody (ab186120) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 3 minutes
ab186120 has not yet been referenced specifically in any publications.