Recombinant Anti-GAK antibody [EPR25327-102] (ab288566)
![Western blot - Anti-GAK antibody [EPR25327-102] (ab288566)](https://www.abcam.com/ps/products/288/ab288566/Images/ab288566-480567-anti-gak-antibody-epr25327102-western-blot-embryonic.jpg "Western blot - Anti-GAK antibody [EPR25327-102] (ab288566)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25327-102] to GAK
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GAK antibody [EPR25327-102]
See all GAK primary antibodies -
Description
Rabbit monoclonal [EPR25327-102] to GAK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HEK-293T and Daudi lysates. IP: Daudi cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25327-102 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 143 kDa.
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 143 kDa. |
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IP
1/30. |
Target
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Function
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. -
Tissue specificity
Ubiquitous. Highest in testis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 C2 tensin-type domain.
Contains 1 J domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus > trans-Golgi network. Cell junction > focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions. - Information by UniProt
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Database links
- Entrez Gene: 2580 Human
- Omim: 602052 Human
- SwissProt: O14976 Human
- Unigene: 369607 Human
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Alternative names
- Auxilin 2 antibody
- Cyclin G associated kinase antibody
- Cyclin-G-associated kinase antibody
see all
Images
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Western blot - Anti-GAK antibody [EPR25327-102] (ab288566)All lanes : Anti-GAK antibody [EPR25327-102] (ab288566) at 1/1000 dilution
Lane 1 :Human wild-type HEK-293T cell line (ab255449)
Lane 2 :Human GAK knockout HEK-293T cell line (ab266417)
Lane 3 : Daudi (Human Burkitt’s lymphoma lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 143 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 59 seconds
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![Immunoprecipitation - Anti-GAK antibody [EPR25327-102] (ab288566)](https://www.abcam.com/ps/products/288/ab288566/Images/ab288566-1-anti-gak-antibody-epr25327102-immunoprecipitation-daudi.jpg)
Immunoprecipitation - Anti-GAK antibody [EPR25327-102] (ab288566)GAK was immunoprecipitated from 0.35 mg Daudi (Human Burkitt’s lymphoma lymphoblast) whole cell lysate 10ug using Anti-GAK antibody [EPR25327-102] at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GAK antibody [EPR25327-102] at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Daudi (Human Burkitt’s lymphoma lymphoblast) whole cell lysate 10ug
Lane 2: ab288566 IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab288566 in Daudi whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
The bands beneath the target band may be caused by degradation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab288566 has not yet been referenced specifically in any publications.