Product nameAnti-GAL4 antibody [EPR12710(B)]
See all GAL4 primary antibodies
DescriptionRabbit monoclonal [EPR12710(B)] to GAL4
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Rat, Human
Does not react with: Mouse
Synthetic peptide within Human GAL4 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P56470
- Purchase matching WB positive control:Recombinant Human GAL4 protein
- HT-29, Human small intestine and rat small intestine lysates. Human colon and colon carcinoma tissue.
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 36 kDa.|
FunctionGalectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
- Information by UniProt
- Antigen NY CO 27 antibody
- Antigen NY-CO-27 antibody
- Antigen NYCO27 antibody
All lanes : Anti-GAL4 antibody [EPR12710(B)] (ab175185) at 1/1000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : Human small intestine lysates
Lane 3 : Rat small intestine lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GAL4 with ab175185 at a 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GAL4 with ab175185 at a 1/250 dilution.
ab175185 has not yet been referenced specifically in any publications.