Product nameAnti-GAL4 antibody [GAL4–8]
See all GAL4 primary antibodies
DescriptionMouse monoclonal [GAL4–8] to GAL4
SpecificityThis antibody can be used to detect both N-terminal and C-terminal tagged Gal4(1–147) and Gal4(1–92) fusion proteins. The clone number has been updated from (3G152) to (GAL4–8) both clone numbers name the same antibody clone.
Tested applicationsSuitable for: ICC/IF, ELISA, WB, ICCmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant fragment, corresponding to amino acids 1-147 of S. cerevisiae GAL4.
EpitopeThe immunoreactive epitope maps within amino acids 1–92 of GAL4.
- N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
Purification notesPurified from ascites.
Light chain typekappa
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab14477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 20610754|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 3 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC||Use at an assay dependent concentration.|
RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
- Gal4p antibody
- GAL81 antibody
- Regulatory protein GAL4 antibody
This product has been referenced in:
- Ren R et al. Visualization of aging-associated chromatin alterations with an engineered TALE system. Cell Res 27:483-504 (2017). Read more (PubMed: 28139645) »
- Chen Y et al. Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons. J Neurosci 30:9199-208 (2010). ICC/IF . Read more (PubMed: 20610754) »