Product nameAnti-GAL4 antibody [GAL4–8]
See all GAL4 primary antibodies
DescriptionMouse monoclonal [GAL4–8] to GAL4
SpecificityThis antibody can be used to detect both N-terminal and C-terminal tagged Gal4(1–147) and Gal4(1–92) fusion proteins. The clone number has been updated from (3G152) to (GAL4–8) both clone numbers name the same antibody clone.
Tested applicationsSuitable for: ICC/IF, ELISA, WB, ICCmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant fragment, corresponding to amino acids 1-147 of S. cerevisiae GAL4.
EpitopeThe immunoreactive epitope maps within amino acids 1–92 of GAL4.
- N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab175185 as a replacement.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Light chain typekappa
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab14477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 20610754|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 3 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC||Use at an assay dependent concentration.|
RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
- Gal4p antibody
- GAL81 antibody
- Regulatory protein GAL4 antibody
This product has been referenced in:
- Ren R et al. Visualization of aging-associated chromatin alterations with an engineered TALE system. Cell Res 27:483-504 (2017). Read more (PubMed: 28139645) »
- Chen Y et al. Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons. J Neurosci 30:9199-208 (2010). ICC/IF . Read more (PubMed: 20610754) »