Overview

  • Product name

    Galactose and Lactose Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    1h 00m
  • Product overview

    Abcam's Galactose and Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (OD570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Galactose Assay Buffer WM 1 x 25ml
    Galactose Enzyme Mix (Lyophilized) Green 1 vial
    Galactose Probe (DMSO solution) Red 1 x 200µl
    Galactose Standard (100nmol/ul) Yellow 1 x 100µl
    HRP (Lyophilized) Purple 1 vial
    Lactase (Lyophilized) Blue 1 vial
  • Research areas

  • Relevance

    Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

Images

  • Example of a Colorimetric standard curve using ab83378.

Protocols

References

ab83378 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Bitte entschuldigen Sie Verspätung meiner Antwort.
Ich habe endlich Nachricht aus dem Labor erhalten:
"If the client is interested only in Lactose measurement, I would highly recommend him to go with the ab83384 kit. If his emphasis is on lactose, but at some point he would like to measure the galactose as well, he can go with the ab83378.

Both kits work on pretty much similar principle, with a minor difference.


The customer can dilute the samples with the assay buffers provided.
Ich hoffe, dies hilft Ihnen weiter

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