Galactose Assay Kit (ab83382)

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Overview

  • Product name

    Galactose Assay Kit

  • Detection method

    Colorimetric/Fluorometric

  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

  • Assay type

    Quantitative

  • Sensitivity

    < 10 nmol/well

  • Assay time

    0h 40m

  • Product overview

    Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Galactose (C6H12O6 MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of a-D-galactose and ß-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Galactose Assay Kit (ab83382)
    Functional Studies - Galactose Assay Kit (ab83382)

    Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.

    Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.

  • Functional Studies - Galactose Assay Kit (ab83382)
    Functional Studies - Galactose Assay Kit (ab83382)

    Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.

  • Functional Studies - Galactose Assay Kit (ab83382)
    Functional Studies - Galactose Assay Kit (ab83382)

     Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Functional Studies - Galactose Assay Kit (ab83382)
    Functional Studies - Galactose Assay Kit (ab83382)

    Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.

  • Functional Studies - Galactose Assay Kit (ab83382)
    Functional Studies - Galactose Assay Kit (ab83382)
    Example of Galactose standard curve obtained with ab83382

Protocols

References

This product has been referenced in:

  • Turpin DL  et al. Intermittent Suckling in Combination with an Older Weaning Age Improves Growth, Feed Intake and Aspects of Gastrointestinal Tract Carbohydrate Absorption in Pigs after Weaning. Animals (Basel) 6:N/A (2016). Read more (PubMed: 27792138) »
See 1 Publication for this product

Publishing research using ab83382? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

I'm wondering if this assay can tell a difference between galactose and galactose-1 phosphate. Can it also tell a difference between galactose and other epimers of glucose?

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Answer

Ab83382 has some cross-reactivity with Galactose-1-phosphate but its extremely low. There is no cross-reactivity with glucose and insignificant cross-reactivity with sucrose has been noticed.

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Question

Thanks a lot for your reply.

Did you get some information in regards to minimal possible rate of the detection of galactosidase (more specific than only < 10 nmol/well that the datasheet indicates).

I think that I wasn't clear: the customer does not intend to measure enzyme activity. He measures galactosidase, but this is not free galactosidase. It is galastosidase that is bound to the protein. Will the kit ab83382 measure bound galactosidase?

Thank you again for your kind support.

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Answer

Thank you for your message
I can confirm that this kit (ab83382: Galactose Assay Kit) will measure only galactose. The detection range which your customer has is not associated with this kit. All we mention on the product datasheet is that this kit is sensitive and quantifies the free galactose only.

Here is a reference for this kit:

David A. Sela et al., Bifidobacterium longum subsp. infantis ATCC 15697 α-Fucosidases Are Active on Fucosylated Human Milk Oligosaccharides, Appl. Envir. Microbiol., Feb 2012; 78: 795 - 803.

Hope this information has been useful for you. Please let me know if you have any other questions.

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Question

Dear Tech Support Team,

Our customer wonders if ab83382 detects free galactose or galactose bound to the protein can be also measured. This customer measures glycosylation rate of the proteins in condition medium.

Thank you for the assistance and reply.

Kind Regards,

Read More
Answer

Thank you for your enquiry.
The ab83382 assay is for galactose. There is not going to be any measurement of enzyme activity in this kit. This kit will measure the total amount of free galactose in your samples.
If you need any further assistance in the future, please do not hesitate to contact me.

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Question

I would like to get the galactose standard in this kit separately. Do you sell it?

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Answer

Thank you for your patience with this request.

The datasheet team added this standard as ab140312. It should be available on the catalogue in a few days.

I hope this information helps. Please contact us with any other questions.

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Question

Phone call requesting information on exactly what the 100 tests provided with this kit encompass. Does it include the standard curve?

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Answer

Thank you for contacting us.


With one Galactose Assay Kit (ab83382) you are provided with enough reagents for a total of 100 tests. As discussed over the phone this does include all the control assays that need to be performed (so taking the 6x2 standard curve tests which need to be performed, this leaves you with a total of 88 tests left.


There is no 96 well plate provided with the kit. This allows you flexibility in choosing which detection system to use with the kit (fluorescence, black plates, clear bottom; luminescence, white plates; colorimeter, clear plate).The fluorometric assay is ˜10 fold more sensitive than the colorimetric assay.


I hope this information has been of help. If you require any further information please do not hesitate to ask.

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