Anti-Galactosidase alpha antibody (ab230924)
Key features and details
- Rabbit polyclonal to Galactosidase alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Galactosidase alpha antibody
See all Galactosidase alpha primary antibodies -
Description
Rabbit polyclonal to Galactosidase alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Galactosidase alpha aa 150 to the C-terminus. (NP_000160.1).
Database link: P06280 -
Positive control
- WB: SW620, BT-474 and HeLa cell extracts. IHC-P: Human adrenal tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Involvement in disease
Defects in GLA are the cause of Fabry disease (FD) [MIM:301500]. FD is a rare X-linked sphingolipidosis disease where glycolipid accumulates in many tissues. The disease consists of an inborn error of glycosphingolipid catabolism. FD patients show systemic accumulation of globotriaoslyceramide (Gb3) and related glycosphingolipids in the plasma and cellular lysosomes throughout the body. Clinical recognition in males results from characteristic skin lesions (angiokeratomas) over the lower trunk. Patients may show ocular deposits, febrile episodes, and burning pain in the extremities. Death results from renal failure, cardiac or cerebral complications of hypertension or other vascular disease. Heterozygous females may exhibit the disorder in an attenuated form, they are more likely to show corneal opacities. -
Sequence similarities
Belongs to the glycosyl hydrolase 27 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 2717 Human
- Omim: 300644 Human
- SwissProt: P06280 Human
- Unigene: 69089 Human
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Alternative names
- AGAL_HUMAN antibody
- Agalsidase alfa antibody
- Alpha D galactosidase A antibody
see all
Images
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All lanes : Anti-Galactosidase alpha antibody (ab230924) at 1/500 dilution
Lane 1 : SW620 cell extract
Lane 2 : BT-474 (human ductal breast epithelial tumor cell line) cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Predicted band size: 49 kDa -
Formalin-fixed, paraffin-embedded human adrenal tissue stained for Galactosidase alpha with ab230924 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Galactosidase alpha (green) using ab230924 at 1/50 dilution in ICC/IF.
The nuclear counter stain is DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230924 has not yet been referenced specifically in any publications.