Key features and details
- Rabbit polyclonal to Galactosidase alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Galactosidase alpha antibody
See all Galactosidase alpha primary antibodies
DescriptionRabbit polyclonal to Galactosidase alpha
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Galactosidase alpha aa 150-429. (NP_000160.1).
GYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIV YSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFN QERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSND LRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAW AVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEW TSRLRSHINPTGTVLLQLENTMQMSLKDLL
Database link: P06280
- WB: SW620, BT-474 and HeLa cell extracts. IHC-P: Human adrenal tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab230924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
Involvement in diseaseDefects in GLA are the cause of Fabry disease (FD) [MIM:301500]. FD is a rare X-linked sphingolipidosis disease where glycolipid accumulates in many tissues. The disease consists of an inborn error of glycosphingolipid catabolism. FD patients show systemic accumulation of globotriaoslyceramide (Gb3) and related glycosphingolipids in the plasma and cellular lysosomes throughout the body. Clinical recognition in males results from characteristic skin lesions (angiokeratomas) over the lower trunk. Patients may show ocular deposits, febrile episodes, and burning pain in the extremities. Death results from renal failure, cardiac or cerebral complications of hypertension or other vascular disease. Heterozygous females may exhibit the disorder in an attenuated form, they are more likely to show corneal opacities.
Sequence similaritiesBelongs to the glycosyl hydrolase 27 family.
- Information by UniProt
- AGAL_HUMAN antibody
- Agalsidase alfa antibody
- Alpha D galactosidase A antibody
All lanes : Anti-Galactosidase alpha antibody (ab230924) at 1/500 dilution
Lane 1 : SW620 cell extract
Lane 2 : BT-474 (human ductal breast epithelial tumor cell line) cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Predicted band size: 49 kDa
Formalin-fixed, paraffin-embedded human adrenal tissue stained for Galactosidase alpha with ab230924 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Galactosidase alpha (green) using ab230924 at 1/50 dilution in ICC/IF.
The nuclear counter stain is DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230924 has not yet been referenced specifically in any publications.