Overview

  • Product name

  • Description

    Goat polyclonal to Galanin
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    HRSFSDKNGLTSK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 51-63 of Galanin (NP_057057.2)

  • Positive control

    • Human Brain (Cerebellum) lysate, Mouse Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 13 kDa.
IHC-FoFr Use a concentration of 0.2 - 0.5 µg/ml.

Target

Images

  • Anti-Galanin antibody (ab99452) at 1 µg/ml + Human Cerebellum Lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 13 kDa

  • Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining Galanin with ab99452 at 0.3 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References

This product has been referenced in:

  • Ippagunta SK  et al. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat Immunol 12:1010-6 (2011). WB . Read more (PubMed: 21892172) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Mesenchymal Stem Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mesenchymal Stem Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Yen You Nick Chen

Verified customer

Submitted Dec 21 2016

Answer

I am very pleased to hear you would like to accept our offer and test ab99452 in IHC. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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