- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-GALE antibody
See all GALE primary antibodies -
DescriptionRabbit polyclonal to GALE
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to a region within amino acids 107 and 326 of Human GALE (UniProt: Q14376).
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Positive control
- A431 and Raji whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
Target
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FunctionCatalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.
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PathwayCarbohydrate metabolism; galactose metabolism.
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Involvement in diseaseDefects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.
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Sequence similaritiesBelongs to the sugar epimerase family.
- Information by UniProt
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Database links
- Entrez Gene: 2582 Human
- Entrez Gene: 74246 Mouse
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: Q8R059 Mouse
- SwissProt: P18645 Rat
- Unigene: 632380 Human
see all -
Alternative names
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
Images
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Western blot - Anti-GALE antibody (ab153824)Anti-GALE antibody (ab153824) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 38 kDa
10% SDS PAGE
Protocols
References
ab153824 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"