Product nameAnti-GALE antibody
See all GALE primary antibodies
DescriptionRabbit polyclonal to GALE
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human GALE aa 220-348 (C terminal).
Database link: Q14376
- SW480, SKOV3, HepG2, U251 and HeLa cell extracts; Mouse and rat liver extracts; A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 38 kDa.|
|ICC/IF||1/10 - 1/100.|
FunctionCatalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.
PathwayCarbohydrate metabolism; galactose metabolism.
Involvement in diseaseDefects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.
Sequence similaritiesBelongs to the sugar epimerase family.
- Information by UniProt
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
All lanes : Anti-GALE antibody (ab210807) at 1/500 dilution
Lane 1 : SW480 cell extract
Lane 2 : SKOV3 cell extract
Lane 3 : HepG2 cell extract
Lane 4 : U251 cell extract
Lane 5 : HeLa cell extract
Lane 6 : Mouse liver extract
Lane 7 : Rat liver extract
Predicted band size: 38 kDa
Immunofluorescent analysis of A549 cells labeling GALE with ab210807 at 1/10 dilution. Blue: DAPI for nuclear staining.
ab210807 has not yet been referenced specifically in any publications.