Overview

  • Product name

  • Description

    Rabbit polyclonal to GALE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human GALE aa 220-348 (C terminal).
    Database link: Q14376

  • Positive control

    • SW480, SKOV3, HepG2, U251 and HeLa cell extracts; Mouse and rat liver extracts; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab210807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 38 kDa.
ICC/IF 1/10 - 1/100.

Target

  • Function

    Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.
  • Pathway

    Carbohydrate metabolism; galactose metabolism.
  • Involvement in disease

    Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.
  • Sequence similarities

    Belongs to the sugar epimerase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ95174 antibody
    • FLJ97302 antibody
    • Galactose 4 epimerase UDP antibody
    • Galactowaldenase antibody
    • galE antibody
    • GALE_HUMAN antibody
    • OTTHUMP00000002991 antibody
    • OTTHUMP00000002994 antibody
    • OTTHUMP00000037931 antibody
    • OTTHUMP00000044857 antibody
    • SDR1E1 antibody
    • short chain dehydrogenase/reductase family 1E member 1 antibody
    • UDP galactose 4 epimerase antibody
    • UDP galactose 4' epimerase antibody
    • UDP glucose 4 epimerase antibody
    • UDP-galactose 4-epimerase antibody
    • UDP-glucose 4-epimerase antibody
    see all

Images

  • All lanes : Anti-GALE antibody (ab210807) at 1/500 dilution

    Lane 1 : SW480 cell extract
    Lane 2 : SKOV3 cell extract
    Lane 3 : HepG2 cell extract
    Lane 4 : U251 cell extract
    Lane 5 : HeLa cell extract
    Lane 6 : Mouse liver extract
    Lane 7 : Rat liver extract

    Predicted band size: 38 kDa

  • Immunofluorescent analysis of A549 cells labeling GALE with ab210807 at 1/10 dilution. Blue: DAPI for nuclear staining.

References

ab210807 has not yet been referenced specifically in any publications.

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