Recombinant Anti-GALE antibody [EPR11088(B)] (ab155997)
![Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/products/155/ab155997/Images/GALE-Primary-antibodies-ab155997-1.jpg "Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11088(B)] to GALE
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-GALE antibody [EPR11088(B)]
See all GALE primary antibodies -
Description
Rabbit monoclonal [EPR11088(B)] to GALE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human GALE (UniProt Q14376).
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Positive control
- HepG2, A375, MCF7 and HeLa cell lysates; Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11088(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
|
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 38 kDa. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. -
Pathway
Carbohydrate metabolism; galactose metabolism. -
Involvement in disease
Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development. -
Sequence similarities
Belongs to the sugar epimerase family. - Information by UniProt
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Database links
- Entrez Gene: 2582 Human
- Entrez Gene: 74246 Mouse
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: Q8R059 Mouse
- SwissProt: P18645 Rat
- Unigene: 632380 Human
see all -
Alternative names
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
Images
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Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)All lanes : Anti-GALE antibody [EPR11088(B)] (ab155997) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
![Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/products/155/ab155997/Images/GALE-Primary-antibodies-ab155997-2.jpg)
Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)Flow cytometric analysis of permeabilized HeLa cells labeling GALE with ab155997 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). -
![Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/products/155/ab155997/Images/ab155997-3-benefits-of-recombinant-antibodies.png)
Anti-GALE antibody [EPR11088(B)] (ab155997)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155997 has been referenced in 3 publications.
- Tran DH et al. Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling. Nat Commun 11:1771 (2020). PubMed: 32286306
- Andres LM et al. Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation. ACS Chem Biol 12:2030-2039 (2017). PubMed: 28541657
- Zhu Y et al. Hepatic GALE Regulates Whole-Body Glucose Homeostasis by Modulating Tff3 Expression. Diabetes 66:2789-2799 (2017). PubMed: 28877911