Anti-GALE antibody [EPR11088(B)] (ab155997)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-GALE antibody [EPR11088(B)]
See all GALE primary antibodies -
DescriptionRabbit monoclonal [EPR11088(B)] to GALE
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide corresponding to residues in Human GALE (UniProt Q14376).
-
Positive control
- HepG2, A375, MCF7 and HeLa cell lysates; Hela cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR11088(B)
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab155997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 38 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IP | 1/10 - 1/100. |
Target
-
FunctionCatalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.
-
PathwayCarbohydrate metabolism; galactose metabolism.
-
Involvement in diseaseDefects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.
-
Sequence similaritiesBelongs to the sugar epimerase family.
- Information by UniProt
-
Database links
- Entrez Gene: 2582 Human
- Entrez Gene: 74246 Mouse
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: Q8R059 Mouse
- SwissProt: P18645 Rat
- Unigene: 632380 Human
see all -
Alternative names
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
Images
-
![Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/datasheet/images/155/ab155997/GALE-Primary-antibodies-ab155997-1.jpg)
Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)All lanes : Anti-GALE antibody [EPR11088(B)] (ab155997) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
![Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/datasheet/images/155/ab155997/GALE-Primary-antibodies-ab155997-2.jpg)
Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)Flow cytometric analysis of permeabilized HeLa cells labeling GALE with ab155997 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
References
ab155997 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"