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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-GALE antibody [EPR11088(B)] (ab155997)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)
  • Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)
  • Anti-GALE antibody [EPR11088(B)] (ab155997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11088(B)] to GALE
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human GALE protein (ab96767)

View more associated products

Overview

  • Product name

    Anti-GALE antibody [EPR11088(B)]
    See all GALE primary antibodies
  • Description

    Rabbit monoclonal [EPR11088(B)] to GALE
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human GALE (UniProt Q14376).

  • Positive control

    • HepG2, A375, MCF7 and HeLa cell lysates; Hela cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11088(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-GALE antibody [EPR11088(B)] - BSA and Azide free (ab249240)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human GALE protein (ab96767)
  • Related Products

    • Recombinant Human GALE protein (ab96767)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.
    • Pathway

      Carbohydrate metabolism; galactose metabolism.
    • Involvement in disease

      Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.
    • Sequence similarities

      Belongs to the sugar epimerase family.
    • Target information above from: UniProt accession Q14376 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2582 Human
      • Entrez Gene: 74246 Mouse
      • Entrez Gene: 114860 Rat
      • Omim: 606953 Human
      • SwissProt: Q14376 Human
      • SwissProt: Q8R059 Mouse
      • SwissProt: P18645 Rat
      • Unigene: 632380 Human
      • Unigene: 247946 Mouse
      • Unigene: 29908 Rat
      see all
    • Alternative names

      • FLJ95174 antibody
      • FLJ97302 antibody
      • Galactose 4 epimerase UDP antibody
      • Galactowaldenase antibody
      • galE antibody
      • GALE_HUMAN antibody
      • OTTHUMP00000002991 antibody
      • OTTHUMP00000002994 antibody
      • OTTHUMP00000037931 antibody
      • OTTHUMP00000044857 antibody
      • SDR1E1 antibody
      • short chain dehydrogenase/reductase family 1E member 1 antibody
      • UDP galactose 4 epimerase antibody
      • UDP galactose 4' epimerase antibody
      • UDP glucose 4 epimerase antibody
      • UDP-galactose 4-epimerase antibody
      • UDP-glucose 4-epimerase antibody
      see all

    Images

    • Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)
      Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)
      All lanes : Anti-GALE antibody [EPR11088(B)] (ab155997) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 38 kDa

    • Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)
      Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)
      Flow cytometric analysis of permeabilized HeLa cells labeling GALE with ab155997 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
    • Anti-GALE antibody [EPR11088(B)] (ab155997)
      Anti-GALE antibody [EPR11088(B)] (ab155997)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab155997? Please let us know so that we can cite the reference in this datasheet.

    ab155997 has been referenced in 3 publications.

    • Tran DH  et al. Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling. Nat Commun 11:1771 (2020). PubMed: 32286306
    • Andres LM  et al. Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation. ACS Chem Biol 12:2030-2039 (2017). PubMed: 28541657
    • Zhu Y  et al. Hepatic GALE Regulates Whole-Body Glucose Homeostasis by Modulating Tff3 Expression. Diabetes 66:2789-2799 (2017). PubMed: 28877911

    Customer reviews and Q&As

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