Recombinant
Recombinant Anti-GALE antibody [EPR11088(B)] (ab155997)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-GALE antibody [EPR11088(B)]
See all GALE primary antibodies -
Description
Rabbit monoclonal [EPR11088(B)] to GALE -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human GALE (UniProt Q14376).
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Positive control
- HepG2, A375, MCF7 and HeLa cell lysates; Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11088(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 38 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. -
Pathway
Carbohydrate metabolism; galactose metabolism. -
Involvement in disease
Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development. -
Sequence similarities
Belongs to the sugar epimerase family. - Information by UniProt
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Database links
- Entrez Gene: 2582 Human
- Entrez Gene: 74246 Mouse
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: Q8R059 Mouse
- SwissProt: P18645 Rat
- Unigene: 632380 Human
see all -
Alternative names
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
Images
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![Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/datasheet/images/155/ab155997/GALE-Primary-antibodies-ab155997-1.jpg)
Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997)All lanes : Anti-GALE antibody [EPR11088(B)] (ab155997) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
![Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)](https://www.abcam.com/ps/datasheet/images/155/ab155997/GALE-Primary-antibodies-ab155997-2.jpg)
Flow Cytometry - Anti-GALE antibody [EPR11088(B)] (ab155997)Flow cytometric analysis of permeabilized HeLa cells labeling GALE with ab155997 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Datasheets and documents
References
This product has been referenced in:
- Andres LM et al. Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation. ACS Chem Biol 12:2030-2039 (2017). Read more (PubMed: 28541657) »
- Zhu Y et al. Hepatic GALE Regulates Whole-Body Glucose Homeostasis by Modulating Tff3 Expression. Diabetes 66:2789-2799 (2017). Read more (PubMed: 28877911) »
