Recombinant Anti-GALE antibody [EPR11089(B)] (ab155277)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11089(B)] to GALE
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-GALE antibody [EPR11089(B)]
See all GALE primary antibodies -
Description
Rabbit monoclonal [EPR11089(B)] to GALE -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
A synthetic peptide corresponding to residues in Human GALE was (UniProt ID: Q14376).
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Positive control
- Human fetal liver, HT29 and HepG2 lysates.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11089(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. -
Pathway
Carbohydrate metabolism; galactose metabolism. -
Involvement in disease
Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development. -
Sequence similarities
Belongs to the sugar epimerase family. - Information by UniProt
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Database links
- Entrez Gene: 2582 Human
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: P18645 Rat
- Unigene: 632380 Human
- Unigene: 29908 Rat
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Alternative names
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
Images
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![Western blot - Anti-GALE antibody [EPR11089(B)] (ab155277)](https://www.abcam.com/ps/products/155/ab155277/Images/GALE-Primary-antibodies-ab155277-1.jpg)
Western blot - Anti-GALE antibody [EPR11089(B)] (ab155277)All lanes : Anti-GALE antibody [EPR11089(B)] (ab155277) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HT29 lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Anti-GALE antibody [EPR11089(B)] (ab155277)](https://www.abcam.com/ps/products/155/ab155277/Images/ab155277-2-benefits-of-recombinant-antibodies.png)
Anti-GALE antibody [EPR11089(B)] (ab155277)
Datasheets and documents
References (0)
ab155277 has not yet been referenced specifically in any publications.
