Anti-Galectin 1 antibody (ab112525)
Key features and details
- Rabbit polyclonal to Galectin 1
- Suitable for: ICC, IHC-Fr, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Galectin 1 antibody
See all Galectin 1 primary antibodies -
Description
Rabbit polyclonal to Galectin 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of Human Galectin 1.
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Positive control
- IHC-P: Human gastric and ovarian cancer tissue, human tonsil tissue IHC-Fr: Human tonsil tissue ICC: U20s cells WB: Hela, MCF-7, COLO-320, A549, 22RV1 and A375 whole cell lysates,
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 2 µg/ml.
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa.
The detection limit for Galectin 1 is approximately 2.5ng/lane under non-reducing and reducing conditions. |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 2 µg/ml. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for Galectin 1 is approximately 2.5ng/lane under non-reducing and reducing conditions. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P09382 Human
- SwissProt: P16045 Mouse
- SwissProt: P11762 Rat
- Unigene: 445351 Human
see all -
Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
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ab112525 staining Galectin 1 in U20S tissue by Immunohistochemistry. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI.
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ab112525 staining Galectin 1 in intestinal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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ab112525 (1 μg/mL) staining Galectin 1 in placenta tissue by Immunohistochemistry (Frozen sections)
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ab112525 staining Galectin 1 in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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ab112525 staining Galectin 1 in gastric cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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All lanes : Anti-Galectin 1 antibody (ab112525) at 0.5 µg/ml
Lane 1 : Hela whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lane 3 : COLO-320 whole cell lysates
Lane 4 : A549 whole cell lysates
Lane 5 : 22RV1 whole cell lysates
Lane 6 : A375 whole cell lysates
Lysates/proteins at 50 µg per lane.
Predicted band size: 15 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab112525 has not yet been referenced specifically in any publications.