Overview

  • Product name
  • Description
    Rabbit polyclonal to Galectin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab25138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use a concentration of 0.5 µg/ml. Using 100 µl/well of antibody solution, allows the detection of 0.2-0.4 ng/well of recombinant human Galectin 1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity
    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities
    Contains 1 galectin domain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Anti-Galectin 1 antibody (ab25138) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 32 kDa, 45 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • ICC/IF image of ab25138 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab25138 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25138, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab25138 staining Galectin 1 in Human umbilical cord blood mescenchymal stem cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 30 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polycloanl (1/2000) was used as the secondary antibody.

    Purple - isotype control, Green - Galectin 1.

    See Abreview

  • ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    Representative images from double stainings of Galectin-1 and CD163. Arrows indicate examples of double positive cells.

  • ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    Representative images from double stainings of Galectin-1 and CD3.

References

This product has been referenced in:
  • Allavena G  et al. A broad-spectrum antibiotic, DCAP, reduces uropathogenic Escherichia coli infection and enhances vorinostat anticancer activity by modulating autophagy. Cell Death Dis 9:780 (2018). Read more (PubMed: 30006504) »
  • Sun L  et al. MIR506 induces autophagy-related cell death in pancreatic cancer cells by targeting the STAT3 pathway. Autophagy 13:703-714 (2017). Read more (PubMed: 28121485) »
See all 15 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
No
Sample
Human Cell (umbilical cord blood mescenchymal stem cell)
Specification
umbilical cord blood mescenchymal stem cell
Gating Strategy
we did not perform the gating step
Preparation
Cell harvesting/tissue preparation method: detachment using trypsin
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Jan 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (293T, Hela)
Specification
293T, Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 24 2013

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.


Having a short look at the two datasheets and particularly at the immunogens, I noticed that ab25138 is directed against the whole galectin-1 protein, whereas ab118851 detects an internal sequence of the protein. ELISA is usually performed under non- reducing and denaturing conditions, so it may be that the relatively small epitope for ab118851 is simply covered- up by thehuge FITC protein. However, I rather gather some more detailed information regarding your protocol before I come to any conclusion.


I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

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Answer

Pas de problème, j'ai donc demandé à notre service comptabilité de mettre en place un avoir, référence CN est ****** (pour le ab94571). Voici les manières dont vous pouvez utiliser cette référence CN: (1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée (2) la faire valoir contre une prochaine facture (3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité. Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB. Concernant l'anti-galectine 1 (ab25138), le numéro de commande de remplacement gratuit de ce produit par uine unité de ab58085 est ********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. L'équipe technique reste à votre disposition pour plus de conseils. Bonne continuation dans votre recherche.

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Answer

Merci pour ces informations. Je suis désolé d'apprendre qu'aucun des 2 anticorps de remplacement n'a fonctionné en western blot sur échantillons humains. Pour des raisons de qualité, j'ai transmis vos données et commentaires au laboratoire afin que des tests supplémentaires soient entrepris pour ces 2 références. Afin de résoudre ce problème le plus rapidement possible, pourriez-vous m'indiquer si vous préférez recevoir un avoir ou un remboursement contre la commande originale ou un remplacement avec des anticorps différents? Pour la détection de la Galectine 1 humaine en western blot, nous avons les 5 anticorps suivants (testés WB et humain) : • ab108389, rabbit monoclonal, www.abcam.com/ab108389 • ab38328, rabbit polyclonal, www.abcam.com/ab38328 • ab114087, goat polyclonal, www.abcam.com/ab114087 • ab58085, mouse monoclonal, www.abcam.com/ab58085 • ab112525, rabbit polyclonal, www.abcam.com/ab112525 Pour la détection de la isocitrate deshydrogenase humaine en western blot, nous avons les 5 anticorps suivants (testés WB et humain) : • ab117976, mouse monoclonal, www.abcam.com/ab117976 • ab113232, rabbit polyclonal, www.abcam.com/ab113232 • ab81653, rabbit polyclonal, www.abcam.com/ab81653 • ab85532, rabbit polyclonal, www.abcam.com/ab85532 • ab117539, rabbit polyclonal, www.abcam.com/ab117539 Merci de me confirmer la résolution qui vous conviendra le mieux.

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Answer

DISCOUNT CODE: xxxxxxxxxxx Expiration date: 14-01-2012 I am very pleased to hear you would like to accept our offer and test ab25138 in Flow Cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab25138. To our knowledge, ab25138 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab25138 now, test it in Flow and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab25138 in Flow. I will then send a discount code. This code must be issued before purchasing ab25138 so please wait for my reply before ordering. 2. Purchase ab25138 either by phone, fax, or online (www.abcam.com). 3. Test it in Flow. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab25138 turns out to be unsuitable for Flow, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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