Anti-Galectin 1 antibody (ab25138)
Key features and details
- Rabbit polyclonal to Galectin 1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Galectin 1 antibody
See all Galectin 1 primary antibodies -
Description
Rabbit polyclonal to Galectin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 1.34% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (3) |
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 3956 Human
- Omim: 150570 Human
- SwissProt: P09382 Human
- Unigene: 445351 Human
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Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
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Anti-Galectin 1 antibody (ab25138) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 32 kDa, 45 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
ICC/IF image of ab25138 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab25138 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25138, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Representative images from double stainings of Galectin-1 and CD163. Arrows indicate examples of double positive cells.
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ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Representative images from double stainings of Galectin-1 and CD3.
Protocols
Datasheets and documents
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Datasheet download
References (32)
ab25138 has been referenced in 32 publications.
- Arnold S et al. Fra-2 overexpression upregulates pro-metastatic cell-adhesion molecules, promotes pulmonary metastasis, and reduces survival in a spontaneous xenograft model of human breast cancer. J Cancer Res Clin Oncol 148:1525-1542 (2022). PubMed: 34693476
- Grabowska K et al. HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages. J Neurovirol 28:99-112 (2022). PubMed: 35175539
- Bai T et al. Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblasts. Stem Cell Res 56:102507 (2021). PubMed: 34454392
- Pihán P et al. Control of lysosomal-mediated cell death by the pH-dependent calcium channel RECS1. Sci Adv 7:eabe5469 (2021). PubMed: 34767445
- Jin XX et al. Galectin-1 expression in the serum and placenta of pregnant women with fetal growth restriction and its significance. BMC Pregnancy Childbirth 21:14 (2021). PubMed: 33407212