Overview

  • Product name
  • Description
    Rabbit polyclonal to Galectin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab25138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use a concentration of 0.5 µg/ml. Using 100 µl/well of antibody solution, allows the detection of 0.2-0.4 ng/well of recombinant human Galectin 1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity
    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities
    Contains 1 galectin domain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Anti-Galectin 1 antibody (ab25138) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 32 kDa, 45 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • ICC/IF image of ab25138 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab25138 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25138, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab25138 staining Galectin 1 in Human umbilical cord blood mescenchymal stem cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 30 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polycloanl (1/2000) was used as the secondary antibody.

    Purple - isotype control, Green - Galectin 1.

    See Abreview

  • ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    Representative images from double stainings of Galectin-1 and CD163. Arrows indicate examples of double positive cells.

  • ab25138 staining Galectin-1 in Human cervical cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/1000 in 1% BSA in PBS) overnight at room temperature. An Alexa Fluor A647-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    Representative images from double stainings of Galectin-1 and CD3.

References

This product has been referenced in:
  • Allavena G  et al. A broad-spectrum antibiotic, DCAP, reduces uropathogenic Escherichia coli infection and enhances vorinostat anticancer activity by modulating autophagy. Cell Death Dis 9:780 (2018). Read more (PubMed: 30006504) »
  • Sun L  et al. MIR506 induces autophagy-related cell death in pancreatic cancer cells by targeting the STAT3 pathway. Autophagy 13:703-714 (2017). Read more (PubMed: 28121485) »
See all 18 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
No
Sample
Human Cell (umbilical cord blood mescenchymal stem cell)
Specification
umbilical cord blood mescenchymal stem cell
Gating Strategy
we did not perform the gating step
Preparation
Cell harvesting/tissue preparation method: detachment using trypsin
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Jan 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (293T, Hela)
Specification
293T, Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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