ab38328 gave a positive signal in the following Human Lysates: Heart, Skeletal Muscle, Kidney (Data not shown), Thymus (Data not shown), HeLa, A431, HL60 (Data not shown), Ramos (Data not shown) and THP1 (Data not shown).
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May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Contains 1 galectin domain.
Secreted > extracellular space > extracellular matrix.
Lanes 1 - 2: Merged signal (red and green). Green - ab38328 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab38328 was shown to recognize Galectin 1 in wild-type HAP1 cells as signal was lost at the expected MW in LGALS1 (Galectin 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. Ab38328 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Galectin 1 antibody (ab38328)
All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa Observed band size: 14 kDa Additional bands at: 34 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Stegemann C et al. Proteomic identification of matrix metalloproteinase substrates in the human vasculature. Circ Cardiovasc Genet6:106-17 (2013).
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Woolley JF & Al-Rubeai M The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnol Bioeng104:590-600 (2009).
Read more (PubMed: 19544303) »