Product nameAnti-Galectin 1 antibody
See all Galectin 1 primary antibodies
DescriptionRabbit polyclonal to Galectin 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human, Chinese hamster
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Orangutan
- Recombinant human Galectin 1 protein (ab50237) can be used as a positive control in WB. ab38328 gave a positive signal in the following Human Lysates: Heart, Skeletal Muscle, Kidney (Data not shown), Thymus (Data not shown), HeLa, A431, HL60 (Data not shown), Ramos (Data not shown) and THP1 (Data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab38328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).Can be blocked with Human Galectin 1 peptide (ab38327).|
FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Lanes 1 - 2: Merged signal (red and green). Green - ab38328 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab38328 was shown to recognize Galectin 1 in wild-type HAP1 cells as signal was lost at the expected MW in LGALS1 (Galectin 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. Ab38328 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Stegemann C et al. Proteomic identification of matrix metalloproteinase substrates in the human vasculature. Circ Cardiovasc Genet 6:106-17 (2013). WB . Read more (PubMed: 23255316) »
- Woolley JF & Al-Rubeai M The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnol Bioeng 104:590-600 (2009). WB ; Chinese hamster . Read more (PubMed: 19544303) »