Product nameAnti-Galectin 1 antibody (Biotin)
See all Galectin 1 primary antibodies
DescriptionRabbit polyclonal to Galectin 1 (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Mouse, Human
highly pure (>98%) recombinant full length Galectin 1 protein (Human).
- This antibody gave a positive signal in the following whole cell lysates: HeLa; U20S; C2C12.
FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: None
Constituents: 0.1% BSA, PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Purification notesab84032 is a sterile filtered antibody solution.
Our Abpromise guarantee covers the use of ab84032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
All lanes : Anti-Galectin 1 antibody (Biotin) (ab84032) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ab84032 has not yet been referenced specifically in any publications.