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    galectin-1-antibody-epr3205-ab108389.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-Galectin 1 antibody [EPR3205] (ab108389)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (10)

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Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
  • Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
  • Anti-Galectin 1 antibody [EPR3205] (ab108389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3205] to Galectin 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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HRP Anti-Galectin 1 antibody [EPR3205] (ab203476)
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Overview

  • Product name

    Anti-Galectin 1 antibody [EPR3205]
    See all Galectin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3205] to Galectin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HL60, K562, and NIH/3T3 cell lysates. Mouse heart, kidney, and spleen tissue lysates. Rat heart and kidney tissue lysates; ICC/IF: NIH/3T3 and K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3205
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • HRP Anti-Galectin 1 antibody [EPR3205] (ab203476)
    • Anti-Galectin 1 antibody [EPR3205] - Low endotoxin, Azide free (ab225540)
    • Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • K-562 whole cell lysate (ab7911)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant human Galectin 1 protein (ab50237)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
ICC/IF
1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
ICC/IF
1/100.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P09382 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3956 Human
    • Entrez Gene: 16852 Mouse
    • Entrez Gene: 56646 Rat
    • Omim: 150570 Human
    • SwissProt: P09382 Human
    • SwissProt: P16045 Mouse
    • SwissProt: P11762 Rat
    • Unigene: 445351 Human
    • Unigene: 43831 Mouse
    • Unigene: 57 Rat
    see all
  • Alternative names

    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)

    Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)

    Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  • Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

    Lane 1 : mouse heart tissue lysate
    Lane 2 : mouse kidney tissue lysate
    Lane 3 : mouse spleen tissue lysate
    Lane 4 : rat heart tissue lysate
    Lane 5 : rat kidney tissue lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Anti-Galectin 1 antibody [EPR3205] (ab108389)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab108389? Please let us know so that we can cite the reference in this datasheet.

ab108389 has been referenced in 10 publications.

  • Chiang MT  et al. Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation. Arterioscler Thromb Vasc Biol 41:331-345 (2021). PubMed: 33147994
  • Chen K  et al. Both serum and tissue Galectin-1 levels are associated with adverse clinical features in neuroblastoma. Pediatr Blood Cancer 65:e27229 (2018). PubMed: 29797641
  • Gao Y  et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). PubMed: 29549328
  • Shahbaz S  et al. CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-ß. PLoS Biol 16:e2006649 (2018). PubMed: 30550561
  • You Y  et al. MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma. Oncotarget 7:57099-57116 (2016). PubMed: 27494859
  • Pereira PM  et al. Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLoS One 9:e95529 (2014). WB, ICC/IF ; Human . PubMed: 24763311
  • Gurel Z  et al. Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy. PLoS One 9:e95561 (2014). WB ; Mouse . PubMed: 24788674
  • Lee YJ  et al. Spatial and temporal expression, and statin responsiveness of galectin-1 and galectin-3 in murine atherosclerosis. Korean Circ J 43:223-30 (2013). WB, IHC-FoFr ; Mouse . PubMed: 23682281
  • Miwa HE  et al. Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer. Glycobiology 23:1477-90 (2013). Mouse . PubMed: 24037315
  • Saint-Lu N  et al. Identification of galectin-1 and galectin-3 as novel partners for von Willebrand factor. Arterioscler Thromb Vasc Biol 32:894-901 (2012). PubMed: 22267483

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Galectin 1 antibody [EPR3205]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
alpha synuclein 3hr
Specification
human
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 30 2015

Question

I wanted to ask whether it is possible to have the aforementioned antibody purified in PBS, in order to couple it directly to a dye.
Thank you in advance,

Read More

Abcam community

Verified customer

Asked on Sep 12 2012

Answer



As this is an Epitomics product, I have forwarded your request to them. They will be in catact with you shortly.

Read More

Abcam Scientific Support

Answered on Sep 12 2012

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