Recombinant Anti-Galectin 1 antibody [EPR3205] (ab108389)
![Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)](https://www.abcam.com/ps/products/108/ab108389/Images/ab108389-121041-anti-galectin-1-antibody-epr3205-western-blot.jpg "Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3205] to Galectin 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Galectin 1 antibody [EPR3205]
See all Galectin 1 primary antibodies -
Description
Rabbit monoclonal [EPR3205] to Galectin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HL60, K562, and NIH/3T3 cell lysates. Mouse heart, kidney, and spleen tissue lysates. Rat heart and kidney tissue lysates; ICC/IF: NIH/3T3 and K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3205 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa. |
|
ICC/IF
1/100. |
Target
-
Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P09382 Human
- SwissProt: P16045 Mouse
- SwissProt: P11762 Rat
- Unigene: 445351 Human
see all -
Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
-
Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)](https://www.abcam.com/ps/products/108/ab108389/Images/ab108389-394077-anti-galectin-1-antibody-epr3205-immunocytochemistry-k562.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)](https://www.abcam.com/ps/products/108/ab108389/Images/ab108389-394078-anti-galectin-1-antibody-epr3205-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
-
![Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)](https://www.abcam.com/ps/products/108/ab108389/Images/ab108389-121040-anti-galectin-1-antibody-epr3205-western-blot.jpg)
Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1 : mouse heart tissue lysate
Lane 2 : mouse kidney tissue lysate
Lane 3 : mouse spleen tissue lysate
Lane 4 : rat heart tissue lysate
Lane 5 : rat kidney tissue lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
![Anti-Galectin 1 antibody [EPR3205] (ab108389)](https://www.abcam.com/ps/products/108/ab108389/Images/ab108389-6-benefits-of-recombinant-antibodies.png)
Anti-Galectin 1 antibody [EPR3205] (ab108389)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab108389 has been referenced in 10 publications.
- Chiang MT et al. Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation. Arterioscler Thromb Vasc Biol 41:331-345 (2021). PubMed: 33147994
- Chen K et al. Both serum and tissue Galectin-1 levels are associated with adverse clinical features in neuroblastoma. Pediatr Blood Cancer 65:e27229 (2018). PubMed: 29797641
- Gao Y et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). PubMed: 29549328
- Shahbaz S et al. CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-ß. PLoS Biol 16:e2006649 (2018). PubMed: 30550561
- You Y et al. MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma. Oncotarget 7:57099-57116 (2016). PubMed: 27494859
- Pereira PM et al. Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLoS One 9:e95529 (2014). WB, ICC/IF ; Human . PubMed: 24763311
- Gurel Z et al. Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy. PLoS One 9:e95561 (2014). WB ; Mouse . PubMed: 24788674
- Lee YJ et al. Spatial and temporal expression, and statin responsiveness of galectin-1 and galectin-3 in murine atherosclerosis. Korean Circ J 43:223-30 (2013). WB, IHC-FoFr ; Mouse . PubMed: 23682281
- Miwa HE et al. Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer. Glycobiology 23:1477-90 (2013). Mouse . PubMed: 24037315
- Saint-Lu N et al. Identification of galectin-1 and galectin-3 as novel partners for von Willebrand factor. Arterioscler Thromb Vasc Biol 32:894-901 (2012). PubMed: 22267483